Abstract
Purpose :
Advanced imaging techniques provide more information about the eye, which advance both our research and diagnosis capabilities. This project aimed at recording and projecting a high-resolution in-vivo 3D hologram of the human eye, with emphasis on retinal layers.
Methods :
An instrument was built to acquire high-resolution holograms of the eye. The instrument consists of a high coherence laser split into a reference and object beams. The interference between the reference beam and the light reflected from the eye was recorded using a low-noise high-sensitivity camera. The vergence of the reference beam was chosen to match that of the feature of interest to obtain reasonable fringe separation on the detector. A second instrument was built to use a reconstruction beam and a liquid crystal spatial light modulator to project the hologram of the eye in space with large magnification. A ground glass diffuser was used as a screen to look at specific planes in the hologram.
Results :
An in-vivo 3D hologram was recorded for one eye where the information for the entire illuminated path was recorded. The vergence of the reference beam matched that for retinal reflections. Several layers of the retina were reconstructed and had enough resolution to show single cells.
Conclusions :
The first in-vivo high-resolution digital holograms of the eye were achieved, capable of resolving single cells on the retina.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.