July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018

Rescue of retinal ganglion cells by human umbilical cord mesenchymal stem cells in a microbead induced ocular hypertension rat model
Author Affiliations & Notes
  • Shangli Ji
    ophthalmology, Aier School of ophthalmology, Changsha, China
  • Zhiyuan Li
    Central South University, Changsha, China
  • Heping Xu
    Aier Eye Institute, Changsha, United Kingdom
  • Jiansu Chen
    ophthalmology, Aier School of ophthalmology, Changsha, China
    Aier Eye Institute, Changsha, United Kingdom
  • Shibo Tang
    ophthalmology, Aier School of ophthalmology, Changsha, China
    Aier Eye Institute, Changsha, United Kingdom
  • Footnotes
    Commercial Relationships   Shangli Ji, None; Zhiyuan Li, None; Heping Xu, None; Jiansu Chen, None; Shibo Tang, None
  • Footnotes
    Support   “555 talent plan” grant from the Changsha National High-Tech Industrial Development Zoom
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 6111. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Shangli Ji, Zhiyuan Li, Heping Xu, Jiansu Chen, Shibo Tang;
      Rescue of retinal ganglion cells by human umbilical cord mesenchymal stem cells in a microbead induced ocular hypertension rat model. Invest. Ophthalmol. Vis. Sci. 2018;59(9):6111.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the potential therapeutic benefits of intravitreally transplanted human umbilical cord mesenchymal stem cells (UC-MSCs) on an animal model of microbead-injection induced ocular hypertension (OHT).

Methods : UC-MSCs were isolated from human umbilical cords and cultured. The OHT model was induced via intracameral injection of polystyrene microbeads of Sprague–Dawley adult rat eyes. Thirty-six healthy adult rats were randomly divided into three groups: normal control, OHT model treated with intravitreal transplantation of UC-MSCs or PBS. Two days after OHT was induced, 5 µl 105 UC-MSCs suspension or PBS were injected into the vitreous cavity of rats. UC-MSCs localization and integration were examined via immunohistochemistry. Neuroprotection was quantified by counting retinal ganglion cells (RGCs) and axons two weeks after transplantation, and the expression levels of glial-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP) were assessed via immunohistochemistry and Western blot, respectively.

Results : Elevated IOP sustained at least three weeks after intracameral microbead injection and the number of β-III-tubulin+ RGCs had significantly declined compared to PBS injected eyes. After intravitreal transplantation, UC-MSCs survived for at least two weeks and predominantly located in the vitreous cavity. However, a proportion of cells migrated into the ganglion cell layer (GCL) of host retina but without differentiation. Intravitreal UC-MSC transplantation resulted in a statistically significant increase of the number of RGCs and axons as well as the expression of GDNF, BDNF, but significant decrease of the GFAP expression.

Conclusions : Intravitreal transplantation of UC-MSCs has revealed neuroprotection in microbead-injection induced OHT, and the effects might be related to the secretion of tropic factors (BDNF and GDNF) and the modulation of glial cell activation.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×