Purchase this article with an account.
Mingming Zhu, Bonnie NK Choy, Jimmy SM Lai; Neuro-protective effect of resveratrol on ischemia/reperfusion-Induced Retinal Injury in mice. Invest. Ophthalmol. Vis. Sci. 2018;59(9):6116.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Retinal ischemia/reperfusion (RIR) injury is a common pathological cause of visual impairment and blindness. Resveratrol is a natural phenolic phytoalexin produced by plants to defend injuries and pathogens. Its benefits of neuroprotection have been found in various neurodegenerative diseases; however, limited evidences were present for RIR injury models. The aim of this study is to explore neuro-protective effect of resveratrol in retinal ischemia-reperfusion(RIR) injury.
The RIR injury model was established by increasing intraocular pressure (IOP) to 95mmHg for 90 minutes by inserting a needle, which was connected to the elevated balanced-salt solution, to the anterior chamber of the right eye of Male C57BL/6J mice. Afterwards, the mice were divided into resveratrol treatment group (n=7, 20mg/kg) and sham treatment group (n=6). The treatment was done by intraperitoneal injection and was initiated on the day of IOP elevation, and repeated daily for 1 week and 4 weeks respectively. Full-field electroretinogram (ERG) was conducted to evaluate retinal function. After scarification, the retina was labelled by Brn-3a and flat-mounted for RGC counting. The independent samples t test was conducted for statistical analysis. A p value less than 0.05 was set as statistical significance.
In this experiment, IOP elevation caused the percentage of RGC loss by 38.43±10.63(%) (p<0.0001) and 40.27±11.19(%) (p<0.0001) at 1 week and 4 weeks respectively. After treatment with resveratrol for 1 week and 4 weeks, the percentage of RGC loss was significantly reduced to 26.45±6.77(%) and 25.17±7.35(%) (p<0.05) respectively. RIR injury decreased b-wave amplitude by 47.38±10.52(%) at 1 week postoperatively. Treated with resveratrol for 1 week reduced percentage of b-wave amplitude to 21.64±13.80% (p<0.05); but the improvement of b-wave amplitude after resveratrol treatment for 4 weeks did not reach the statistical significance.
Resveratrol was effective in protecting RGC from degeneration induced by RIR injury in mice. In addition, resveratrol might be promising for preventing inner nuclear layer interneuron damage from RIR injury
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
This PDF is available to Subscribers Only