July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Hesperidin has an anti-inflammatory effect after NMDA-induced retinal injury in mice
Author Affiliations & Notes
  • Taimu Sato
    Tohoku University, Sendai, Miyagi, Japan
  • Kota Sato
    Tohoku University, Sendai, Miyagi, Japan
  • Toru Nakazawa
    Tohoku University, Sendai, Miyagi, Japan
  • Footnotes
    Commercial Relationships   Taimu Sato, None; Kota Sato, DAIICHI SANKYO Co., Ltd. (F), Japan Tobacco Inc. (F), Kowa Co., Ltd. (F), Santen Pharmaceutical Co., Ltd. (F), Senju Pharmaceutical Co., Ltd. (F), TOPCON CORPORATION (F), WAKAMTO Co., Ltd. (F); Toru Nakazawa, Alcon Pharma Co., Ltd. (F), DAIICHI SANKYO Co., Ltd. (F), Japan Tobacco Inc. (F), Kowa Co., Ltd. (F), NIDEK CO., LTD. (F), Otsuka Pharmaceutical Co., Ltd. (F), Santen Pharmaceutical Co., Ltd. (F), Senju Pharmaceutical Co., Ltd. (F), TOPCON CORPORATION (F), WAKAMOTO Co., Ltd. (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 6120. doi:
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      Taimu Sato, Kota Sato, Toru Nakazawa; Hesperidin has an anti-inflammatory effect after NMDA-induced retinal injury in mice. Invest. Ophthalmol. Vis. Sci. 2018;59(9):6120.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the anti-inflammatory properties of hesperidin, a polyphenol, and its protective effect against NMDA injury in the retina of mice.

Methods : This study used 8-12-week-old male C57BL/6J mice. NMDA (30 nmol) was injected into the vitreous cavity to induce excitotoxic retinal damage, with or without 17% hesperidin. Six to twenty-four hours after NMDA injection, the eyes were collected and the microglia were stained with an anti-Iba1 antibody. The transcriptional levels of proinflammatory mediators were measured with qRT-PCR. To determine the effect of hesperidin on inflammatory cytokine-mediated activation of NF-κB, RGC-5/NF-κB/luc stable cells were established. The cells were pretreated with hesperidin for 2 hours, and then treated with TNFα (20 ng/mL) or vehicle control for 4 hours. Luciferase activity was measured using the ONE-Glo luciferase assay system kit.

Results : The number of Iba1-positive microglia was significantly increased in the retinas 6 to 24 h after NMDA injection (6 h: 6.5 ± 2.2 cells/mm, 12 h: 7.7 ± 1.5 cells/mm, 24 h: 7.0 ± 1.3 cells/ mm), in comparison with non-treated control retinas (1.4 ± 1.1 cells/mm). NMDA injection significantly induced the gene expression of TNF, IL-1b, IL-6, and MCP-1. Co-injection of hesperidin with NMDA significantly suppressed the NMDA-induced mRNA expression of these genes 6 h after injection. Treatment with 0.5% or 5% hesperidin significantly suppressed TNFα-induced relative luciferase activity (0% hesperidin: 34.99 ± 0.87, 0.5% hesperidin: 30.53 ± 0.90, 5% hesperidin: 13.24 ± 0.24) in an NF-κB luciferase assay.

Conclusions : Hesperidin may have a role in ameliorating the inflammatory response after NMDA injury in the mouse retina, via the suppression of NF-κB activation.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

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