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Taimu Sato, Kota Sato, Toru Nakazawa; Hesperidin has an anti-inflammatory effect after NMDA-induced retinal injury in mice. Invest. Ophthalmol. Vis. Sci. 2018;59(9):6120.
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To investigate the anti-inflammatory properties of hesperidin, a polyphenol, and its protective effect against NMDA injury in the retina of mice.
This study used 8-12-week-old male C57BL/6J mice. NMDA (30 nmol) was injected into the vitreous cavity to induce excitotoxic retinal damage, with or without 17% hesperidin. Six to twenty-four hours after NMDA injection, the eyes were collected and the microglia were stained with an anti-Iba1 antibody. The transcriptional levels of proinflammatory mediators were measured with qRT-PCR. To determine the effect of hesperidin on inflammatory cytokine-mediated activation of NF-κB, RGC-5/NF-κB/luc stable cells were established. The cells were pretreated with hesperidin for 2 hours, and then treated with TNFα (20 ng/mL) or vehicle control for 4 hours. Luciferase activity was measured using the ONE-Glo luciferase assay system kit.
The number of Iba1-positive microglia was significantly increased in the retinas 6 to 24 h after NMDA injection (6 h: 6.5 ± 2.2 cells/mm, 12 h: 7.7 ± 1.5 cells/mm, 24 h: 7.0 ± 1.3 cells/ mm), in comparison with non-treated control retinas (1.4 ± 1.1 cells/mm). NMDA injection significantly induced the gene expression of TNF, IL-1b, IL-6, and MCP-1. Co-injection of hesperidin with NMDA significantly suppressed the NMDA-induced mRNA expression of these genes 6 h after injection. Treatment with 0.5% or 5% hesperidin significantly suppressed TNFα-induced relative luciferase activity (0% hesperidin: 34.99 ± 0.87, 0.5% hesperidin: 30.53 ± 0.90, 5% hesperidin: 13.24 ± 0.24) in an NF-κB luciferase assay.
Hesperidin may have a role in ameliorating the inflammatory response after NMDA injury in the mouse retina, via the suppression of NF-κB activation.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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