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MINA MIZUKAMI, Ji-Ae Ko, Diah Gemala Ibrahim, Hideaki Okumichi, Yoshiaki Kiuchi; Functional Analysis of MANF in Retinal Ganglion Cells by Oxidative Stress. Invest. Ophthalmol. Vis. Sci. 2018;59(9):6146.
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© ARVO (1962-2015); The Authors (2016-present)
The neuroprotection of retinal ganglion cells is important for development of new drugs for glaucoma. We have now examined the effects of mesencephalic astrocyte derived neutrophic factor (MANF) on the retinal ganglion cells survival by oxidative stress.
Primary retinal ganglion cells from 4-5days rats after birth were cultured. And, the cells were treated with 500 mM hydrogen peroxide (H2O2), at the same time, stimulated by MANF. After cultivation for indicated time, using RT-PCR and immunoblot analysis, the expression levels of the several survival markers of retinal ganglion cells were studied. Also, the extension of neurites of retinal ganglion cells after culture was examined by Immunofluorescence analysis.
Immunoblot and Immunofluorescence analysis were indicated the down regulated expression levels of the several survival markers of RGC, also extension of neurites were decreased in RGC with 500 mM H2O2. But, in additional MANF, we found blocked down-regulated expression of neural markers and extension of neurites by oxidative stress in RGC. So, the stimulation with MANF was blocked the damage from oxidative stress in RGC.
These results suggest that MANF may play an important role in the survival of RGC with oxidative stress. In brief, it is possible that MANF is important key factor in neuroprotection of RGC.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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