July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Characterization of corneal sensory nerve activity in young and adult guinea pigs
Author Affiliations & Notes
  • Juana Gallar
    Instituto de Neurociencias, Universidad Miguel Hernandez-CSIC, San Juan de Alicante, ALICANTE, Spain
  • Kamila Mizerska
    Instituto de Neurociencias, Universidad Miguel Hernandez-CSIC, San Juan de Alicante, ALICANTE, Spain
    Weill Cornell Medicine, New York, New York, United States
  • Carolina Luna García
    Instituto de Neurociencias, Universidad Miguel Hernandez-CSIC, San Juan de Alicante, ALICANTE, Spain
  • Susana Quirce
    Instituto de Neurociencias, Universidad Miguel Hernandez-CSIC, San Juan de Alicante, ALICANTE, Spain
  • M Carmen Acosta
    Instituto de Neurociencias, Universidad Miguel Hernandez-CSIC, San Juan de Alicante, ALICANTE, Spain
  • Footnotes
    Commercial Relationships   Juana Gallar, None; Kamila Mizerska, None; Carolina Luna García, None; Susana Quirce, None; M Carmen Acosta, None
  • Footnotes
    Support  Arrest Blindness, GA No. 667400, Horizon 2020 Program, European Commission, and in part by SAF2014-54518-C3-1-R and SAF2017-83674-C2-1-R, Spanish Ministry of Economy and Competitiveness and ERDF, European Union.
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 154. doi:
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    • Get Citation

      Juana Gallar, Kamila Mizerska, Carolina Luna García, Susana Quirce, M Carmen Acosta; Characterization of corneal sensory nerve activity in young and adult guinea pigs. Invest. Ophthalmol. Vis. Sci. 2018;59(9):154.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To analyze the activity of corneal sensory nerve fibers in young and adult guinea pigs (1-18 months old).

Methods : The spontaneous and stimulus evoked nerve activity of corneal sensory nerve fibers in response to mechanical (von Frey filaments), thermal (cooling the bath solution from 34oC to 20oC) and chemical stimulation (CO2 gas pulses of 30s) were recorded extracellularly from nerve terminals or axons (ciliary nerve filaments) from ex-vivo eyes of guinea pigs of different ages (young: 1-4 months old; young-adult: 8-9 months; adult: 15-18 months). For mechano- and polymodal nociceptors, mechanical threshold (MT) and percentage of incidence of spontaneous activity (SA) were determined. For polymodal nociceptors impulse response to CO2 was also measured. For cold thermoreceptors, ongoing activity (OA) at basal temperature (34oC), cold threshold (CTH) and peak response (PR) to cold stimulus were studied.

Results : No significant changes were found in corneal nerve activity from corneas of young and young-adult guinea pigs. Activity recorded from adult corneas was different compared with young corneas. Mechanonociceptors had lower MT values. Polymodal nociceptors had slightly higher MT, impulse response to CO2 and incidence of SA. Cold thermoreceptors showed significant differences in their ongoing activity and response to cold stimulation (see Table).

Conclusions : The characteristics of the spontaneous and stimulus-evoked activity of corneal sensory nerves are similar in young (1-4 months old) and young-adult animals (8-9 months old). Activity of mechano- and polymodal nociceptors was modified in adult animals (15-18 months old), while activity of cold thermoreceptors appears to be significantly lower compared with young animals.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

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