July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Variation of potential pain biomarkers in saliva of patients after Advanced Surface Ablation (ASA) refractive technique
Author Affiliations & Notes
  • Eva Sobas Abad
    Ophthalmologhy, IOBA, Valladolid, Valladolid, Spain
    Enfermería, Universidad de Valladolid, Valladolid, Valladolid, Spain
  • Amanda Vazquez
    Ophthalmologhy, IOBA, Valladolid, Valladolid, Spain
  • Sebastian Videla
    Ciencias Experimentales y de la Salud. Facultad de Ciencias de la Salud y de la Vida, Universidad Pompeu Fabra, Barcelona, Barcelona, Spain
  • Roberto Reinoso
    Ophthalmologhy, IOBA, Valladolid, Valladolid, Spain
    Networking Research Center on Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN, Valladolid, Spain
  • Itziar Fernandez
    Ophthalmologhy, IOBA, Valladolid, Valladolid, Spain
    Networking Research Center on Bioengineering, Biomaterials and Nanomedicine , CIBER-BBN, Valladolid, Spain
  • Miguel J Maldonado
    Ophthalmologhy, IOBA, Valladolid, Valladolid, Spain
    Medicina, Universidad de Valladolid, Valladolid, Spain
  • Jose-Carlos Pastor
    Ophthalmologhy, IOBA, Valladolid, Valladolid, Spain
    Medicina, Universidad de Valladolid, Valladolid, Spain
  • Footnotes
    Commercial Relationships   Eva Sobas Abad, None; Amanda Vazquez, None; Sebastian Videla, None; Roberto Reinoso, None; Itziar Fernandez, None; Miguel Maldonado, None; Jose-Carlos Pastor, None
  • Footnotes
    Support  Laboratorios Dr. Esteve S.A.
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5773. doi:
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      Eva Sobas Abad, Amanda Vazquez, Sebastian Videla, Roberto Reinoso, Itziar Fernandez, Miguel J Maldonado, Jose-Carlos Pastor; Variation of potential pain biomarkers in saliva of patients after Advanced Surface Ablation (ASA) refractive technique. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5773.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Analysis biomarkers in saliva can be an objective method to quantify acute pain in a clinical model of post-surgical pain after corneal refractive surgery. Validation of this hypothesis in a clinical sample would validated this proposed clinical model as reliable, quantitative, and repetitive to test new pain management strategies and protocols

Methods : A multicenter, prospective, cohort study, based on 32 caucasian patients between 18 and 60 years-old, free from ocular pain of any origin, who were treated with alcohol-assisted surface ablation with epithelial removal (ASA) in both eyes were included.
Pain intensity was evaluated by Visual Analogue Scale (VAS) and Numeric Pain Rating Scale (NPRS) before and after surgery. Comorbidities (photophobia, burning, tearing, and foreign-body sensation), Hospital Anxiety/Depression (HAD) questionnaire, Quality of Life (QoL) questionnaire, and Visual Function 25 (VF25) questionnaire were gathered before and at 6h after surgery. Saliva was collected with the passive secretion method at basal day (V0), before the surgery (V1) and at 2 (V2) and 24 hours (V3) after surgery. Salivary cortisol, α-amylase (sAA), secretory IgA (sIgA), testosterone, and soluble fraction of receptor II of TNFα (sTNFαRII) concentrations were analyzed.
Baseline characteristics were summarized with standard descriptive statistics, and descriptive analyses were carried out. Analysis of variance (ANOVA) with repeated measures was used to evaluate mean differences over time. Functional Data Analysis (FDA) was used for analyzing the temporal evolution of VAS scores. P values <0.05 were considered statistically significant

Results : Level of sIgA in V2 was significantly higher than in V1 (p 0.0012) and V3 (p 0.0013). Level of sTNFαRII in V2 was significantly higher than in V1 (p 0.0001) and V3 (p 0.0049). Level of cortisol in V0 was significantly lower than in V1 (p<0.0001) and V2 (p 0.0071). Level of sAA in V0 was significantly lower than in V2 (p<0.0001) and V2 (p 0.0071). In the remaining biomarkers no statistically significant variations were found

Conclusions : Salivary sIgA and sTNFαRII show an increase related to pain intensity. Thus ASA could be a useful novel human model of acute post-operative pain for testing new analgesic protocols

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

Evolution of sIgA concentration

Evolution of sIgA concentration

 

Evolution of sTNFαRII concentration

Evolution of sTNFαRII concentration

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