July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Quantitative research of VEGF-A, VEGF-B and PIGF in vitreous and serum in angiogenic ocular disorders
Author Affiliations & Notes
  • Joana Mesquita
    CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal
  • João Paulo Castro de Sousa
    Faculty of Medical Sciences, Universidade da Beira Interior, Covilhã, Portugal
    Department of Ophthalmology, Centro Hospitalar de Leiria, Leiria, Portugal
  • Sara Vaz-Pereira
    Department of Ophthalmology, Hospital de Santa Maria, Lisbon, Portugal
    Department of Ophthalmology, Faculty of Medicine, Universidade de Lisboa, Lisbon, Portugal
  • Arminda Neves
    Department of Ophthalmology, Centro Hospitalar de Leiria, Leiria, Portugal
  • Luís Passarinha
    CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal
    Faculty of Medical Sciences, Universidade da Beira Interior, Covilhã, Portugal
  • Cândida Tomaz
    CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Covilhã, Portugal
  • Footnotes
    Commercial Relationships   Joana Mesquita, Alimera Sciences (E); João Paulo Castro de Sousa, None; Sara Vaz-Pereira, None; Arminda Neves, None; Luís Passarinha, None; Cândida Tomaz, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3085. doi:
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    • Get Citation

      Joana Mesquita, João Paulo Castro de Sousa, Sara Vaz-Pereira, Arminda Neves, Luís Passarinha, Cândida Tomaz; Quantitative research of VEGF-A, VEGF-B and PIGF in vitreous and serum in angiogenic ocular disorders. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3085.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Angiogenesis is responsible for neovascularization. Angiogenic ocular conditions are a leading cause of vision loss. Vascular endothelial growth factors (VEGFs) play a main role in pathological angiogenesis in retinal diseases. VEGF-A, VEGF-B and PIGF were investigated in vitreous and serum of patients with diabetic retinopathy (DR), age-related macular degeneration (AMD), retinal vein occlusion (RVO) and compared with a control group with no neovascular pathology.

Methods : Serum and vitreous samples were collected from 96 patients undergoing vitrectomy: DR (n=63), RVO (n=2), AMD (n=2) and control group (n=29). Cytokines were quantified by ELISA. Optical coherence tomography (OCT) was evaluated for central retinal thickness and macular volume (MV). Results were cross between growth factors and OCT outcomes. This study adhered to the tenets of the Declaration of Helsinki; all patients gave their informed consent.

Results : DR patients revealed vitreous high values (mean±standard deviation pg/mL) of: VEGF-A (370.04±588.62 pg/mL), VEGF-B (88.18±239.13) and PIGF (70.00±39.24). In AMD, serum and vitreous VEGF-A had the highest values (27.23±9.93 vs 9.75±4.92, respectively). In RVO the highest values were vitreous VEGF-A and B, (502.97±539.69 and 295.15±316.56, respectively). A comparison between vitreous levels in neovascular disorders and control demonstrated a statistical difference: VEGF-A (343.30±543.82 vs 7.04±1.35; p=0.050), VEGF-B (93.95±237.61 vs 14.32±7.08; p=0.004) and PIGF (70.00±39.24 vs 46.88±10.14; p=0.007). The comparison between proliferative diabetic retinopathy patients (PDR) and non-PDR (NPDR) showed statistical significant high levels for vitreous: VEGF-A (p = 0.003), VEGF-B (p = 0.001), PIGF (p = 0.009) and serum: VEGF-A (p = 0.028), VEGF-B (p = 0.021), PIGF (p = 0.039). A strong correlation was found between vitreous VEGF-A and B (r=0.959, p≤ 0.001) and serum VEGF-A and B (r=0.716, p=0.001) in DR patients. The correlation between vitreous VEGF-B and MV in DR was moderate (r=0.547, p=0.003).

Conclusions : The results confirm the overexpression of these cytokines in neovascular disorders. In DR patients, vitreous VEGF levels increase with the progression of the disease, being lower in NPDR and higher in PDR patients. The strong correlation between vitreous and serum VEGF-A and VEGF-B suggests a simultaneous pathological increase of these cytokines in DR.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

 

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