July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Preventive effects of (-)-epicatechin on retina and pigmented epithelium impaired by sodium iodate (NaIO3)
Author Affiliations & Notes
  • Bani Antonio-Aguirre
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • Luis Enrique Niembro-Muñoz
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • Xavier P Guillonneau
    UMR-S 968 INSERM UPMC, Institut de la Vision, Paris, France
  • Israel Ramirez-Sanchez
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
    Department of Medicine, University California San Diego , San Diego, California, United States
  • Andric Christopher Perez-Ortiz
    School of Public Health, Yale University, New Haven, Connecticut, United States
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • Alvaro Rendon
    UMR-S 968 INSERM UPMC, Institut de la Vision, Paris, France
  • Esmeralda Lira-Romero
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • Lidia Elena Llacsahuanga-Allcca
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • Martha Janneth Peralta-Ildefonso
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • David Jimenez-Collado
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • Francisco Javier Estrada-Mena
    School of Medicine, Universidad Panamericana, Mexico City, Mexico
  • Footnotes
    Commercial Relationships   Bani Antonio-Aguirre, None; Luis Niembro-Muñoz, None; Xavier Guillonneau, None; Israel Ramirez-Sanchez, None; Andric Perez-Ortiz, None; Alvaro Rendon, None; Esmeralda Lira-Romero, None; Lidia Elena Llacsahuanga-Allcca, None; Martha Peralta-Ildefonso, None; David Jimenez-Collado, None; Francisco Estrada-Mena, None
  • Footnotes
    Support  Universidad Panamericana Grant "Fomento a la Investigación UP 2017" (UP-CI-2017-CS-MX-01)
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 351. doi:
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      Bani Antonio-Aguirre, Luis Enrique Niembro-Muñoz, Xavier P Guillonneau, Israel Ramirez-Sanchez, Andric Christopher Perez-Ortiz, Alvaro Rendon, Esmeralda Lira-Romero, Lidia Elena Llacsahuanga-Allcca, Martha Janneth Peralta-Ildefonso, David Jimenez-Collado, Francisco Javier Estrada-Mena; Preventive effects of (-)-epicatechin on retina and pigmented epithelium impaired by sodium iodate (NaIO3). Invest. Ophthalmol. Vis. Sci. 2018;59(9):351.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Oxidative stress (OS)-induced damage, and prolonged inflammatory responses are prominent pathophysiological mechanisms underlying geographic atrophy (GA) development. (-)-Epicatechin (epi) is an antioxidant flavonoid that appears to ameliorate OS by improving mitochondrial parameters and decreasing inflammatory cytokines. Here, we evaluated the effectiveness of epi in a NaIO3-induced retinal degeneration model, as a potential prophylactic strategy for GA.

Methods : Equal number of wild-type (WT) C57BL/6J mice (n = 30) were randomly assigned to an intervention, sham-treated or control group. The intervention and control group received 1 mg/kg/day of epi q24h PO diluted in a vehicle (H2O, pH 5-6). Shams-treated received only the vehicle. After 15 days of treatment, intervention and sham-treated mice were injected with 30 mg/kg of NaIO3 diluted in PBS on the right retro-orbital venous sinus and were followed by an additional 5 days. Controls were PBS-injected. We planned to perform indirect immunofluorescence (IF) for OS markers (PPRAγ, pATK) on flat-mount retinas and retinal pigment epithelium (RPE)-choroids. Also, western-blot for SOD2 and Gpx1, and qPCR of key inflammatory genes in vitro (IL-1β, CCL2, VEGF, TNF-α). RPE-choroids were stained with phalloidin (red). RPE degeneration ratio was obtained by fluorescence slide scanning and analysis.

Results : RPE degeneration ratio was significantly decreased in epi-fed mice compared to sham (p = 0.0018). Gene expression analysis in vitro, showed a rapid up-regulation of inflammatory cytokines in NaIO3 exposed retinas [e.g. IL-1β Epi(-)/NaIO3(+) 1.132, Epi(+)/NaIO3(+) 0.8223 fold-induction, p = 0.0571]. This effect was ameliorated by epi-treatment. Additionally, epi-fed mice and exposed to NaIO3 had lower SOD2 protein expression than the sham-treated group [Epi (-)/NaIO3 (+)].

Conclusions : Taken altogether, our preliminary results might be suggestive of an early prophylactic benefit with dietary supplementation of (-)-epicatechin in mice. This might be impactful for GA patients. Further corroboration in other animal models is warranted.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

Flat-mount RPE-choroid indirect immunofluorescence Phalloidin (red). A. Control group. B. Sham-treated group. C. Intervention group.

Flat-mount RPE-choroid indirect immunofluorescence Phalloidin (red). A. Control group. B. Sham-treated group. C. Intervention group.

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