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tao guo; Changes in Aqueous Humor Levels of TGFβ2 and SFRP1 in Open-Angle and Angle-Closure Glaucomas. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3517.
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© ARVO (1962-2015); The Authors (2016-present)
To assess levels of transforming growth factor β2 (TGFβ2) and secreted frizzled-related protein 1 (SFRP1) in the aqueous humor(AH) of eyes with different types of glaucoma.
The AH samples were collected from 126 eyes of 105 patients, who were divided into the following six groups: cataract patients serving as controls, primary open angle glaucoma(POAG), chronic angle closure glaucoma(CACG), primary angle closure suspects(PACS), acute angle-closure glaucoma (AACG) with normal intraocular pressure(IOP), and AACG with high IOP. At the time of cataract or glaucoma surgery, a small amount of aqueous was withdrawn. The concentration of TGFβ2 and SFRP1 were measured by enzyme-linked immunosorbent assay . Comparison among groups was performed using one-way analysis of variance (ANOVA), followed by Dunnett’s test. The association between two parameters was assessed by linear regression analysis.
The concentration of active TGFβ2 in AH of eyes with POAG (465.9 ± 33.8 pg/mL) was significantly higher than that in control (cataract) eyes (345.2 ± 24.8 pg/mL) (P = 0.008). Interestingly, levels of TGFβ2 in AH of eyes with AACG normal IOP (242.8 ± 29.5 pg/mL) was lower than control (cataract) eyes (P < 0.05). There was no significant change in concentration of TGFβ2 between control (cataract) eyes and PACS (336.0 ± 54.3 pg/mL) (P = 0.88), with eyes of AACG with high IOP (394.6 ± 33.6 pg/mL) (P = 0.25), with CACG eyes (344.6 ± 37.4 pg/mL) (P = 0.99).Levels of SFRP1 in AH of AACG with high IOP eyes (2.19 ± 0.10 ng/mL) were found to be elevated when comparing to those of cataract controls (1.37 ± 0.18 ng/mL) (P = 0.04). In the AH samples from patients with AACG with normal IOP, the concentration of SFRP1 (1.53 ± 0.18 ng/mL) (P = 0.65) was lower than that with controls, but the difference was not significant. The concentration of SFRP1 in the AH of eyes with POAG (1.88 ± 0.10 ng/mL) (P = 0.52), PACS (1.84 ± 0.18 ng/mL) (P=0.85) and CACG (1.71 ± 0.13 ng/mL) (P = 0.31), were not significantly different from that in eyes with controls. There was no statistically significant correlation between the concentration of active TGFβ2 and SFRP1 in the AH.
Our results indicate that levels of TGFβ2 and SFRP1 may play an important role in the pathogenesis of glaucomas.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
Levels of active TGFβ2 and SFRP1 in AH
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