July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Evaluation of the Protective Role of Melatonin on H2O2-induced Injury in Human Corneal Epithelial Cells
Author Affiliations & Notes
  • Mengliang Wu
    Ophthalmology, Eye and ENT hospital, Fudan University, Shanghai, China
  • Yan Wang
    Ophthalmology, Eye and ENT hospital, Fudan University, Shanghai, China
  • Jing Han
    Ophthalmology, Eye and ENT hospital, Fudan University, Shanghai, China
  • Tingting Shao
    Ophthalmology, Eye and ENT hospital, Fudan University, Shanghai, China
  • Footnotes
    Commercial Relationships   Mengliang Wu, None; Yan Wang, None; Jing Han, None; Tingting Shao, None
  • Footnotes
    Support  This research was supported by the National Science Foundation for Young Scientists of China (No. 81100636)
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3866. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Mengliang Wu, Yan Wang, Jing Han, Tingting Shao; Evaluation of the Protective Role of Melatonin on H2O2-induced Injury in Human Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3866.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the anti-oxidant and anti-inflammatory effects of Melatonin (MLT) against H2O2-Induced injury in human corneal epithelial cells (HCECs) and the mechanism.

Methods : HCECs were pre-incubated with different concentrations of MLT (5µM, 50µM and 500µM) before H2O2 stimulus. Cell viability was evaluated by a cell counting kit-8 assay. Cell apoptosis was assessed by TUNEL assay. Intracellular reactive oxygen species (ROS) was measured by fluorometric analysis using 2’,7’-dichlorofluorescin diacetate (DCFH-DA). Western blot analysis assessed NLRP3 and caspase-1 protein expression. Bioactive IL-1β as well as IL-18 release was assessed by ELISA. Real-time PCR was used to evaluate NLRP3, caspase-1, IL-1β and IL-18 mRNA levels.

Results : Melatonin significantly improved cell viability and decreased apoptosis in HCECs. MLT reduced the expression of IL-1β and Il-18 by inhibiting the activation of NLRP3 and caspase-1 induced by H2O2. Generation of ROS was also reduced by MLT application in HCECs.

Conclusions : Melatonin shows protective and anti-inflammatory effects against H2O2-induced injury likely by its anti-oxidant role and inhibiting ROS-NLRP3-IL-1β axis.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

 

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×