July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Erythropoietin Protects Retinal Pigment Epithelium in Experimental Diabetic Retinopathy
Author Affiliations & Notes
  • Chaoyang Zhang
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Hai Xie
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Yiting Yang
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Qian Yang
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Haibin Tian
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Lixia Lu
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Weiye Li
    Department of Ophthalmology, Drexel University College of Medicine, Philadelphia, Pennsylvania, United States
  • Jingfa Zhang
    Department of Ophthalmology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
  • Guo-Tong Xu
    Department of Ophthalmology of Shanghai Tenth People’s Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Footnotes
    Commercial Relationships   Chaoyang Zhang, None; Hai Xie, None; Yiting Yang, None; Qian Yang, None; Haibin Tian, None; Lixia Lu, None; Weiye Li, None; Jingfa Zhang, None; Guo-Tong Xu, None
  • Footnotes
    Support  National Natural Science Foundation of China (81570852, 31201084 and 81370999), Shanghai Science and Technology Committee Grant (17ZR1431300), Shanghai Health Bureau Scientific Research Grant (201640229), Shanghai Pujiang Program (15PJ1408700), National Key Basic Research Program of China (2013CB967501, 2015CB964601, 2016YFA0101302, 2013CB967101) and Shanghai East Hospital Grant ZJ2014-ZD-002.
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5359. doi:
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    • Get Citation

      Chaoyang Zhang, Hai Xie, Yiting Yang, Qian Yang, Haibin Tian, Lixia Lu, Weiye Li, Jingfa Zhang, Guo-Tong Xu; Erythropoietin Protects Retinal Pigment Epithelium in Experimental Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5359.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : This study was to explore the possible protective mechanism(s) of EPO on retinal pigment epithelial cells (RPE) in diabetic rat retinas.

Methods : The Sprague-Dawley rats were rendered diabetes with intraperitoneal injection of streptozotocin, followed by intravitreal injection of EPO after 2 hours; 2 and 4 weeks later, the permeability of outer blood-retinal barrier was examined by using FITC-dextran leakage assay. The glyoxal-treated ARPE-19 cells, incubated with EPO or soluble EPO receptor, were studied for cell viability, barrier function, etc. The expressions of ZO-1, occludin, VEGFR2, HIF-1α, MAPKs and AKT were examined with Western blot and immunofluorescence.

Results : The leakage of FITC-dextran was detected mainly in outer nuclear layer in 2-week diabetic rat retinas and became more obvious in 4-week diabetic rat retinas. The leakage was largely ameliorated in EPO-treated diabetic rats. The protein expressions of ZO-1 and occludin in the RPE-Bruch’s membrane choriocapillaris complex were decreased, while HIF-1α and JNK pathways were increased, significantly in 4-week diabetic rats, which were reversed by EPO treatment. The in vitro study with ARPE-19 cells also confirmed above changes and the protective effect of EPO.

Conclusions : EPO could protect RPE cells and maintain its barrier integrity through upregulation of ZO-1 and occludin in diabetic rats. The protective mechanism(s) of EPO might be through its downregulation of HIF-1α and JNK pathways, thus protecting outer blood-retinal barrier.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

 

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