July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Using Grapes as a Magic Bullet to Fight Against Free Radicals in the Eye: Application for Cataract Prevention
Author Affiliations & Notes
  • Hongli Wu
    Pharmaceutical Sciences, UNT System College of Pharmacy, Fort Worth, Texas, United States
    North Texas Eye Research Institute, University of North Texas Health & Science Center, Fort Worth, Texas, United States
  • Xiaobin Liu
    Pharmaceutical Sciences, UNT System College of Pharmacy, Fort Worth, Texas, United States
  • Christy Xavier
    Pharmaceutical Sciences, UNT System College of Pharmacy, Fort Worth, Texas, United States
  • Duen-Shian Wang
    Pharmaceutical Sciences, UNT System College of Pharmacy, Fort Worth, Texas, United States
  • Princess Ananti
    Pharmaceutical Sciences, UNT System College of Pharmacy, Fort Worth, Texas, United States
  • Yang Liu
    North Texas Eye Research Institute, University of North Texas Health & Science Center, Fort Worth, Texas, United States
  • Footnotes
    Commercial Relationships   Hongli Wu, None; Xiaobin Liu, None; Christy Xavier, None; Duen-Shian Wang, None; Princess Ananti, None; Yang Liu, None
  • Footnotes
    Support  California Table Grape Commission Grant RP20011
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 5646. doi:
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      Hongli Wu, Xiaobin Liu, Christy Xavier, Duen-Shian Wang, Princess Ananti, Yang Liu; Using Grapes as a Magic Bullet to Fight Against Free Radicals in the Eye: Application for Cataract Prevention. Invest. Ophthalmol. Vis. Sci. 2018;59(9):5646.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The main purpose of this study is to investigate if grape powder could protect against in vivo ultraviolet (UV) radiation-induced cataract and to study its mechanism of action and identify its targets in the lens.

Methods : The grape powder was provided by the California Table Grape Commission (CTGC). C57BL/6J mice were feed with the regular diet, regular diet supplemented with glucose and fructose, or the grape diet (regular diet supplemented with 5%, 10%, and 15% grape powder) for 3 months. The animals were then exposed to 20.6 kJ/m2 UV radiation for 15 min to induce cataracts. Two days later, the degree of the cataract and lens morphology was evaluated under dissecting microscope.Glutathione (GSH), free protein thiol (PSH), and protein glutathionylation (PSSG) levels were measured to reflect the oxidative markers. Finally, we also examined the effects of grape powder on the nuclear factor erythroid 2–related factor 2 (Nrf2) pathway and its downstream antioxidant genes in the lens.

Results : We found that 15% grape powder diet could significantly inhibit the onset as well as the severity of UV-induced cataracts. All mice in the regular diet control group developed severe epithelial and superficial anterior subcapsular cataracts two days after the UV radiation. On the other hand, grape powder diet in a dose-dependent manner prevented the lens from UV radiation-induced cataract progression. In the 15% grape powder diet group, the majority of lenses remained largely transparent. The GSH and PSH levels were much higher in the 15% grape powder diet group compared with that of the regular diet control group. The accumulation of PSSG, a marker for protein thiol oxidation, was largely inhibited in the 15% grape powder diet group. Interestingly, we also found that Nrf2 and its downstream targets including SOD, catalase, thioredoxin (Trx), and glutaredoxin 1 (Grx1) were significantly elevated in regular diet control groups due to the UV exposure. However, this UV-induced Nrf2 activation was largely inhibited in all three grape powder diet groups.

Conclusions : Grape powder dose-dependently protected the lens from UV radiation-induced cataract development in mice. Its protective effects may involve directly regulating endogenous Nrf2 and its downstream target detoxifying/antioxidant genes, including SOD2, catalase, Trx1, and Grx1.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

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