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Wangyuan Liu, Jinhui Dai; The Relation of Light Spectral Composition and Dopamine D1 and D2 Receptors in Lens Induced Myopia in Guinea Pigs. Invest. Ophthalmol. Vis. Sci. 2018;59(9):681.
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© ARVO (1962-2015); The Authors (2016-present)
The balance of dopamine D1 and D2 receptors in retinal has been recognized as an important factor in myopia development. The goal of our research is to explore whether different light spectral composition could change the expression of D1 and D2 receptors in lens induced myopia.
Guinea pigs (n= 54, four weeks of age) were divided into three groups (n= 18 each) reared under LED lighting environments of short-wavelength (430nm±10nm, 800lux, SL), middle-wavelength (530±10nm, 800lux, ML) and broad-band white light (4000K, 800lux, WL) with -5 diopter (D) lenses buckled in front of right eyes. Refraction error, corneal curvature and axial length were measured by retinoscopy, keratometry and A-scan ultrasonography every 2 weeks. Guinea pigs were sacrificed after 4 weeks of light treatment. Retinal D1 receptors(D1R) and D2 receptors(D2R) expressions were determined by simple western and quantitative real-time polymerase chain reaction(PCR). Differences between the two eyes of the same animals in each group were statistically compared by paired t test. Differences between different groups were compared by one-way ANOVA. Differences were defined as significant at P<0.05.
Myopia were induced in all three groups after 2 weeks of light intervention(p<0.01). After 4 weeks, the mean differences between lens-induced and fellow eyes of SL, WL and ML groups were -4.17±2.24D, -5.06±2.8D and -7.20±3.69D respectively. Significant difference between SL and ML groups was found after 4 weeks of intervention(p=0.01). In both PCR and simple western, no difference was found in retinal D1R and D2R expression between lens induced and fellow eye or among three groups. However, the D2R/D1R ratio between lens induced eye and fellow eye was found significantly different among three groups (p=0.02). In SL group, transcription level of D2R/D1R ratio in lens induced eyes was 0.64±0.33 times lower than in fellow eyes. In WL and ML group, D2R/D1R ratios in lens induced eyes were 1.03±0.47 and 1.44±0.35 times higher respectively. The same trend of D2/D1 ratio was also found in protein level (SL 0.58±0.27, WL 0.99±0.20 and ML 1.1±0.42) and was also significantly different(p=0.03).
Light spectral composition change could alter the D2R/D1R ratio in lens induced myopic eye in guinea pigs.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
Corneal cirvature, axial length and refraction
D2/D1 Ratio in PCR, Simple Western and OD/OS
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