July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Bilateral intraocular pressure (IOP) changes in a non human primate (NHP) microbead model of chronic IOP elevation: Can both eyes achieve similar elevations for therapeutics evalution?
Author Affiliations & Notes
  • Anita Chan
    Ophthalmology, Singapore National Eye Centre, Singapore, Singapore
    Ophthalmology, Singapore Eye Research Institute, Singapore, Singapore
  • Myoe Naing Lynn
    Ophthalmology, Singapore Eye Research Institute, Singapore, Singapore
  • Sai Bo Bo Tun
    Ophthalmology, Singapore Eye Research Institute, Singapore, Singapore
  • Veluchamy A Barathi
    Ophthalmology, Singapore Eye Research Institute, Singapore, Singapore
    Academic, Duke NUS Medical School, Singapore, Singapore
  • Tin Aung
    Ophthalmology, Singapore National Eye Centre, Singapore, Singapore
    Ophthalmology, National University of Singapore, Singapore, Singapore
  • Footnotes
    Commercial Relationships   Anita Chan, None; Myoe Lynn, None; Sai Bo Bo Tun, None; Veluchamy Barathi, None; Tin Aung, None
  • Footnotes
    Support  NMRC/TA/0026/2014 Neuroglobin in a Primate Experimental Model of Glaucoma: A Novel Neuroprotectant in Glaucoma
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 2001. doi:https://doi.org/
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      Anita Chan, Myoe Naing Lynn, Sai Bo Bo Tun, Veluchamy A Barathi, Tin Aung; Bilateral intraocular pressure (IOP) changes in a non human primate (NHP) microbead model of chronic IOP elevation: Can both eyes achieve similar elevations for therapeutics evalution?. Invest. Ophthalmol. Vis. Sci. 2018;59(9):2001. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The primary aim is to compare bilateral intraocular pressures (IOP) in a microbead model of chronic experimental glaucoma (EG) over a period of 40weeks and to determine if bilateral IOP elevations will be detriment to general well being. This is to address if bilateral IOP elevations may 1) reduce the number of animals used for future studies and 2) give similar IOP changes if induced simultaneously.

Methods : Experimental procedures were conducted in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and Singhealth institutional IACUC approval for simultaneous bilateral eye IOP induction was obtained based on our previous preliminary data demonstrating unilateral NHP IOP elevation did not have significant changes in social behavior and general health. Bilateral eyes of 6 adult (age, 5 years) and 5 juvenile (age, 2years) Cynomolgus macaque monkeys (Macaca Fascicularis) received IOP elevation using repeated sterile polystyrene microbeads (Triton Technologies, 15um) injections into the anterior chamber (AC). Repeated injections was given until IOP elevation >25mmHg was achieved. The 11 NHP were monitored for 40 weeks with humane endpoints monitored.

Results : All 11 NHP did not show any change in social behavior and maintained good body weight and heath. Bilateral IOP elevations were sustained after week 7 from first injection, with an average of 8±SD 2 weekly injections (range 4-10 repeated weekly injections). No difference in the mean number of injection was needed between the juvenile and adult NHPs. No further injections were required for the 40 week period. There was no statistical significance (p=0.76) between right and left mean IOP elevation from week 6 to 40 (Table 1).

Conclusions : Bilateral chronic moderate IOP elevation from 6-40 weeks does not result in change in NHP social behavior, body weight and health and may be useful to reduce the number of animals used for research. Simultaneous induction of IOP can achieve similar bilateral IOP elevations and may be useful for the study of therapeutic intervention.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

Table 1: Mean IOP elevation in Right (OD) and Left (OS) eyes show no statistical difference (p=0.76).

Table 1: Mean IOP elevation in Right (OD) and Left (OS) eyes show no statistical difference (p=0.76).

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