July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Full shape crystalline lens geometrical changes with age from 3-D OCT images in vivo and ex vivo
Author Affiliations & Notes
  • Eduardo Martinez-Enriquez
    Instituto de Optica "Daza de Valdés", Consejo Superior de Investigaciones Científicas (IO, CSIC), Madrid, Madrid, Spain
  • Ashik Mohamed
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, Telangana, India
    Brien Holden Vision Institute, Sydney, New South Wales, Australia
  • Marco Ruggeri
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Miriam Velasco-Ocana
    Instituto de Optica "Daza de Valdés", Consejo Superior de Investigaciones Científicas (IO, CSIC), Madrid, Madrid, Spain
  • Siobhan Williams
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Bianca Maceo Heilman
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Alberto De Castro
    Instituto de Optica "Daza de Valdés", Consejo Superior de Investigaciones Científicas (IO, CSIC), Madrid, Madrid, Spain
  • Pablo Perez-Merino
    Instituto de Optica "Daza de Valdés", Consejo Superior de Investigaciones Científicas (IO, CSIC), Madrid, Madrid, Spain
  • N. Geetha Sravani,
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, Telangana, India
  • Virender Sangwan
    Ophthalmic Biophysics, L V Prasad Eye Institute, Hyderabad, Telangana, India
  • Jean-Marie A Parel
    Brien Holden Vision Institute, Sydney, New South Wales, Australia
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Robert C. Augusteyn
    Brien Holden Vision Institute, Sydney, New South Wales, Australia
    School of Optometry and Vision Science, The University of New South Wales, Sydney, New South Wales, Australia
  • Arthur Ho
    Brien Holden Vision Institute, Sydney, New South Wales, Australia
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Fabrice Manns
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, Florida, United States
  • Susana Marcos
    Instituto de Optica "Daza de Valdés", Consejo Superior de Investigaciones Científicas (IO, CSIC), Madrid, Madrid, Spain
  • Footnotes
    Commercial Relationships   Eduardo Martinez-Enriquez, US20170316571A1 (P); Ashik Mohamed, None; Marco Ruggeri, None; Miriam Velasco-Ocana, None; Siobhan Williams, None; Bianca Maceo Heilman, None; Alberto De Castro, None; Pablo Perez-Merino, None; N. Geetha Sravani,, None; Virender Sangwan, None; Jean-Marie Parel, None; Robert C. Augusteyn, None; Arthur Ho, None; Fabrice Manns, None; Susana Marcos, US20170316571A1 (P), WO /2012/146811 (P)
  • Footnotes
    Support  NEI Grants: R01EY021834, F31EY021444 (Maceo), P30EY14801 (Center Grant); the Florida Lions Eye Bank; Research to Prevent Blindness; Australian Federal Government CRC Program (Vision CRC); Drs. KR Olsen and ME Hildebrandt, Drs. Raksha Urs and Aaron Furtado, the Henri and Flore Lesieur Foundation (JMP); Hyderabad Eye Research Foundation, European Research Council ERC-AdG 294099, FIS2014-56643-R and CSIC iCoop Program.
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 268. doi:
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      Eduardo Martinez-Enriquez, Ashik Mohamed, Marco Ruggeri, Miriam Velasco-Ocana, Siobhan Williams, Bianca Maceo Heilman, Alberto De Castro, Pablo Perez-Merino, N. Geetha Sravani,, Virender Sangwan, Jean-Marie A Parel, Robert C. Augusteyn, Arthur Ho, Fabrice Manns, Susana Marcos; Full shape crystalline lens geometrical changes with age from 3-D OCT images in vivo and ex vivo. Invest. Ophthalmol. Vis. Sci. 2018;59(9):268.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Quantifying the geometrical changes of the human crystalline lens with age is essential for the design of solutions for age-related disorders such as cataract and presbyopia. We estimated the full shape geometry of the crystalline lens ex vivo and in vivo using optical coherence tomography (OCT)

Methods : 3-D OCT images were acquired from isolated crystalline lenses obtained from human donor eyes (RIEB, Hyderabad, India, n=17; TFS Bank, Barcelona, Spain, n=33) ex vivo and subjects in vivo in a maximally accommodated state (IO-CSIC, Madrid, Spain, n=58), using two custom-developed spectral domain OCT systems, provided with distortion correction routines. Ages ranged from 5-71 years & 21-87 years in the ex vivo and in vivo samples, respectively. Lens volume (VOL), surface area (LSA), thickness (LT), diameter (DIA), and equatorial plane position (EPP) were calculated using custom-developed automatic algorithms, both ex vivo and in vivo using a new methodology that extrapolates realistically the information visible through the pupil (Martinez-Enriquez et al. IOVS 2016). In vivo and ex vivo estimates were analysed as a function of age, and linear regression models were compared

Results : In the ex vivo lenses, VOL and LSA increased linearly with age at rates of 1.19 mm3/year (r=0.85, p<0.01) and 0.69 mm2/year (r=0.75, p<0.01) respectively. LT and EPP showed a biphasic trend, decreasing rapidly in the first 20 years (m=-0.039 mm/year, r=-0.78, p<0.01 for LT; m=-0.028mm/year, r=-0.81, p<0.01 for EPP) and increasing slowly afterwards (m=0.018 mm/year, r=0.82, p<0.01 for LT, m=0.006 mm/year, r=0.49, p<0.01 for EPP). DIA increased rapidly up to 15 years (m=0.23 mm/year, r=0.92, p=0.01) and slowly afterwards (m=0.01 mm/year, r=0.43, p<0.01). The mean absolute difference between ex vivo and in vivo linear regressions within the same age range (21-71 y.o) was below 12 mm3 for VOL, 17 mm2 for LSA, 0.3 mm for DIA and 0.07 mm for EPP. The slopes’ differences were 0.01 mm3/year, 0.08 mm2/year, 0.002 mm/year for VOL, LSA and DIA respectively

Conclusions : 3-D OCT measurements with dedicated algorithms allow ex vivo and in vivo quantification of the full shape of the crystalline lens. The similarity of the lens dimensions and age-related trends measured in vivo and ex vivo reflect the validity of the methodology. All the analyzed parameters ex vivo changed significantly with age, with a biphasic trend for LT and EPP

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

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