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Bharat Kumar, Rohin Dasari, Matthew Aaron Reilly; Lens Capsule Strain Modulates Lens Epithelial Cell Proliferation. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3032.
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© ARVO (1962-2015); The Authors (2016-present)
The specific causes of lens growth throughout an individual’s lifetime is unknown. We tested the hypothesis that lens capsule strain would modulate lens epithelial cell (LEC) proliferation using an experimental in vitro model simulating the accommodative processes. Confirming the hypothesis would indicate that the mechanical forces affecting the lens during accommodation contribute towards the growth of the lens.
Paired porcine lenses were dissected such that the ciliary body and a ring of sclera were still attached. One of the lenses was mounted to a silicone sheet and cultured under a null strain condition. The paired lens was mounted similarly but was stretched at the equator to 12% static strain. After culturing for 24 hours, the LECs were isolated and counted using a hemocytometer; the results were normalized by the null strain counts to give the relative viability index (RVI). The LECs were then fixed and stained to quantify the fraction of proliferating cells, or proliferation index (PI), using a flow cytometer.
The RVI of LECs in the statically strained condition was 1.29, indicating a 29% increase in LEC population in the static group compared to the null group (Figure 1). The difference was statistically significant (p = 0.019). The mean PI of lenses in the null group was 8.38% while the PI of lenses cultured in the static group was 26.92%, indicating an increase in LEC proliferation rate in the static group compared to the null group (Figure 2). The difference was statistically significant (p = 0.038).
These data indicate that mechanically stretching the lens results in an increase in the total LEC population as well as the rate of proliferation and may be a contributing factor in driving the growth of the lens. Future studies will examine the effects of a cyclic strain regime compared to the static and null strain conditions.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
The relative viability index of LEC cultured under the null (left) and static (right) strain conditions
The net proliferation index of LEC cultured under the null (left) and static (right) strain conditions
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