Investigative Ophthalmology & Visual Science Cover Image for Volume 59, Issue 9
July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Corneal Changes in Patients Treated with Rose Bengal Photodynamic Antimicrobial Therapy for Severe Infectious Keratitis
Jaime D. Martinez; Sander R Dubovy; Guillermo Amescua; Pedro Monsalve; Xiao Yi Zhou; Alejandro Arboleda; Heather Ann Durkee; Mariela C Aguilar; Nidhi Relhan; Neda Nikpoor; Harry W Flynn; Darlene Miller; Jean-Marie A Parel
Author Affiliations & Notes
  • Jaime D. Martinez
    Bascom Palmer Eye Institute , Miami, Florida, United States
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Sander R Dubovy
    Florida Lions Ocular Pathology Laboratory, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Bascom Palmer Eye Institute , Miami, Florida, United States
  • Guillermo Amescua
    Bascom Palmer Eye Institute , Miami, Florida, United States
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Pedro Monsalve
    Florida Lions Ocular Pathology Laboratory, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Bascom Palmer Eye Institute , Miami, Florida, United States
  • Xiao Yi Zhou
    Florida Lions Ocular Pathology Laboratory, Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Bascom Palmer Eye Institute , Miami, Florida, United States
  • Alejandro Arboleda
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Heather Ann Durkee
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Mariela C Aguilar
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Nidhi Relhan
    Bascom Palmer Eye Institute , Miami, Florida, United States
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Neda Nikpoor
    Bascom Palmer Eye Institute , Miami, Florida, United States
  • Harry W Flynn
    Bascom Palmer Eye Institute , Miami, Florida, United States
  • Darlene Miller
    Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, Miami, Florida, United States
    Bascom Palmer Eye Institute , Miami, Florida, United States
  • Jean-Marie A Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    Bascom Palmer Eye Institute , Miami, Florida, United States
  • Footnotes
    Commercial Relationships   Jaime Martinez, None; Sander Dubovy, None; Guillermo Amescua, None; Pedro Monsalve, None; Xiao Yi Zhou, None; Alejandro Arboleda, None; Heather Durkee, None; Mariela Aguilar, None; Nidhi Relhan, None; Neda Nikpoor, None; Harry Flynn, None; Darlene Miller, None; Jean-Marie Parel, None
  • Footnotes
    Support  The Janet K. and Edward D. Robson Foundation, Florida Lions Eye Bank, Drs. KR Olsen and ME Hildebrandt, Drs. Raksha Urs and Aaron Furtado, Brien Holden Vision Institute, NIH Center Grant P30EY14801, an unrestricted grant from Research to Prevent Blindness, and the Henri and Flore Lesieur Foundation (JMP).
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 529. doi:
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      Jaime D. Martinez, Sander R Dubovy, Guillermo Amescua, Pedro Monsalve, Xiao Yi Zhou, Alejandro Arboleda, Heather Ann Durkee, Mariela C Aguilar, Nidhi Relhan, Neda Nikpoor, Harry W Flynn, Darlene Miller, Jean-Marie A Parel; Corneal Changes in Patients Treated with Rose Bengal Photodynamic Antimicrobial Therapy for Severe Infectious Keratitis. Invest. Ophthalmol. Vis. Sci. 2018;59(9):529.

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Abstract

Purpose : To describe corneal changes in patients who were treated with rose bengal photodynamic antimicrobial therapy (PDAT) as an adjunct treatment for infectious keratitis (Amescua et al. Cornea. 2017 Sep;36(9):1141-1144).

Methods : Corneal tissue histopathology was examined on four patients who underwent rose bengal PDAT as an adjunct treatment for infectious keratitis and subsequent optical corneal graft once the infection and inflammation resolved. Corneal thickness was measured on histology sections. Corneal tissue histopathology findings were correlated with slit-lamp and Anterior Segment Optical Coherence Tomography (AS-OCT) images taken pre- and post- rose bengal PDAT.

Results : In all cases, corneal tissue histopathology showed a demarcation line on the stromal tissue ranging from 30 to 220 µm from the epithelium. Total corneal tissue mean thickness was 682 µm (range 450 to 940±177.2). In two cases, alpha smooth muscle actin antibody was negative, and in the other two cases had partial positive stromal staining. In one case a clear demarcation line is present post- rose bengal PDAT on both narrow beam slit-lamp and AS-OCT images, at a depth of 220 and 210 µm, respectively.

Conclusions : Corneal tissue histopathology provides information about the wound healing respons to rose bengal PDAT that will be useful in understanding effect of this novel treatment. Corneal stromal demarcation line may be useful in determining the depth of rose bengal PDAT which may help guide future treatments for different stages of progressive infectious keratitis.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

Cornea changes of Case 1 visualized with slit-lamp, histopathology, and AS-OCT. Narrow beam slit-lamp images of (a) pre-and (b) post-rose bengal PDAT showing demarcation line (arrows). (c) Histopathology showing changes in the anterior corneal stroma above the demarcation line (arrows). (d) AS-OCT images showing demarcation line (arrows) post-rose bengal PDAT.
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Cornea changes of Case 1 visualized with slit-lamp, histopathology, and AS-OCT. Narrow beam slit-lamp images of (a) pre-and (b) post-rose bengal PDAT showing demarcation line (arrows). (c) Histopathology showing changes in the anterior corneal stroma above the demarcation line (arrows). (d) AS-OCT images showing demarcation line (arrows) post-rose bengal PDAT.

Cornea changes of Case 1 visualized with slit-lamp, histopathology, and AS-OCT. Narrow beam slit-lamp images of (a) pre-and (b) post-rose bengal PDAT showing demarcation line (arrows). (c) Histopathology showing changes in the anterior corneal stroma above the demarcation line (arrows). (d) AS-OCT images showing demarcation line (arrows) post-rose bengal PDAT.

Cornea changes of Case 1 visualized with slit-lamp, histopathology, and AS-OCT. Narrow beam slit-lamp images of (a) pre-and (b) post-rose bengal PDAT showing demarcation line (arrows). (c) Histopathology showing changes in the anterior corneal stroma above the demarcation line (arrows). (d) AS-OCT images showing demarcation line (arrows) post-rose bengal PDAT.
View OriginalDownload Slide

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Copyright © 2025 Association for Research in Vision and Ophthalmology.
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