Abstract
Purpose :
The aim of our study was to investigate if dopamine and/or serotonin signaling is abnormal in our setting of mouse myopias and the models could be used to study therapeutic effects of transplanted cells producing these transmitters
Methods :
Refractive development was measured in BL/6 mice, and albino, rag2-/- immune deficient BL6 mice from 3 weeks of age, and both FDM and LIM models were used in each of them. The refraction was measured using infrared refractometer, while the MRI have been used to measure ocular biometry. The presence of selected dopamine (D1, D4) and serotonin receptors (5HT2A, 5HT2B) in the eye was evaluated by qRT-PCR, while the concentration of dopamine and serotonin in eye tissues was measured using HPLC. We evaluated three ocular tissues known to be involved in eye refraction and myopia development: retina, sclera and cornea.
Results :
LIM and FDM were successfully induced in wild-type BL/6 and immunodeficient rag2-/- mice; the refractive status of deprived eyes is significantly different from fellow eyes and the results are similar between models and mice (Figure 1). MRI showed elongation of vitreous chamber depth (VCD) in all mouse models. No significant changes in retinal, scleral, and corneal dopamine, DOPAC, and 5HT levels were detected in deprived eyes. However, we find difference in expression of dopaminergic and serotoninergic expression between myopic and fellow eyes. For D1 receptor, there was interaction of model of myopia, mouse strain, myopia induction and type of tissue. Interestingly, out of four observed differences, three concerned sclera: in FDM model induced in Bl6 mice there was decrease of dopamine in sclera, while in the same model but in Rag2 mice the effect was opposite, as well as in LIM model induced in Bl6 mice, and in retina of LIM in rag2 mice. There was observed decrease of 5HT2B receptor in myopic eye of Rag2 mice sclera, and it was particularly evidenced in LIM model (Figure 2). There is also correlation between D1 and D4 receptors and VCD.
Conclusions :
There are abnormalities in dopamine and serotonin signaling in myopia models in our setting, so the models could be used to test cell therapies to prevent or even treat myopia.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.