Abstract
Purpose :
To explore the effect of mechanical strain on human scleral fibroblasts (HSFs) and compared cell behaviors of HSFs from distinct regions.
Methods :
Primary HSFs were cultivated using a digestive protocel. FX-5000T was used to perform mechanical strain in vitro. We applied 10%, 0.5Hz mechanical strain in vitro. Cell behaviors of peripapillary and periphery HSFs were compared after the strain. Edu imaging, CCK-8 assay and cell cycle flow cytometry were conducted to analyze cell proliferation ability. For cell apoptosis, flow cytometry, caspase 3 activity and western blot were performed. Immunofluorescence, qPCR and western blot were used to investigate cell differentiation. Migration assay was also performed.
Results :
Under the mechanical strain of 10%, 0.5Hz for 24 hours/After mechanical strain, the proliferation ability, cell apoptosis and migration rate of peripapillary HSFs did not have a significant change. The expression of α-SMA slightly decreased. However, increased cell proliferation and migration ability, attenuated cell apoptosis and more expression of α-SMA were shown in the periphery HSFs under the same condition.
Conclusions :
Mechanical strain affected cell behaviors of HSFs. The different performance of cells from distinct regions may suggest familial linages of HSFs, probably induced by mechanical strain.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.