July 2018
Volume 59, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2018
Mechanical Strain Induces Distinct Human Scleral Fibroblast Lineages: Differential Roles in Cell Proliferation, Apoptosis, Migration and Differentiation
Author Affiliations & Notes
  • Chen Qiu
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, Shanghai, China
  • Shaohong Qian
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, Shanghai, China
  • Xing-Huai Sun
    Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, Shanghai, China
  • Footnotes
    Commercial Relationships   Chen Qiu, None; Shaohong Qian, None; Xing-Huai Sun, None
  • Footnotes
    Support  NNSFC 81430007
Investigative Ophthalmology & Visual Science July 2018, Vol.59, 3539. doi:
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      Chen Qiu, Shaohong Qian, Xing-Huai Sun; Mechanical Strain Induces Distinct Human Scleral Fibroblast Lineages: Differential Roles in Cell Proliferation, Apoptosis, Migration and Differentiation. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3539.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To explore the effect of mechanical strain on human scleral fibroblasts (HSFs) and compared cell behaviors of HSFs from distinct regions.

Methods : Primary HSFs were cultivated using a digestive protocel. FX-5000T was used to perform mechanical strain in vitro. We applied 10%, 0.5Hz mechanical strain in vitro. Cell behaviors of peripapillary and periphery HSFs were compared after the strain. Edu imaging, CCK-8 assay and cell cycle flow cytometry were conducted to analyze cell proliferation ability. For cell apoptosis, flow cytometry, caspase 3 activity and western blot were performed. Immunofluorescence, qPCR and western blot were used to investigate cell differentiation. Migration assay was also performed.

Results : Under the mechanical strain of 10%, 0.5Hz for 24 hours/After mechanical strain, the proliferation ability, cell apoptosis and migration rate of peripapillary HSFs did not have a significant change. The expression of α-SMA slightly decreased. However, increased cell proliferation and migration ability, attenuated cell apoptosis and more expression of α-SMA were shown in the periphery HSFs under the same condition.

Conclusions : Mechanical strain affected cell behaviors of HSFs. The different performance of cells from distinct regions may suggest familial linages of HSFs, probably induced by mechanical strain.

This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.

 

 

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