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Xiang-Run Huang, Ye Z. Spector, Jianzhong Qiao; Effects of the Location of Optic Nerve Transection on the Retinal Nerve Fiber Layer Reflectance. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3741. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
The animal model of optic nerve transection (ONT) is often used to study pathophysiological mechanisms of neurodegenerative diseases. This study reports the effects of the location of ON transection on the reflectance of the retinal nerve fiber layer (RNFL).
Normal Wistar rats were used in this study. After anesthesia an incision was made in the superior conjunctiva of an eye to allow gentle outward retraction of the globe using a fine forceps. The muscle cone was entered and the optic nerve (ON) was exposed. The exposed ON was then cut at approximately 0.5 mm, 2 mm or 3 mm from the ON exit of the eye. The eyeball was removed followed by isolation of the retina from the pigment epithelium. The retina was then perfused in warm, oxygenated physiological solution. Reflectance images of the RNFL were taken regularly for at least 1.5 hours at a wavelength of 500 nm. Reflectance of nerve fiber bundles and retinal areas between bundles (gaps) were calculated.
Twenty retinas of 10 rats were studied. Transection at the immediate exit (0.5 mm) of the ON caused significant decline of the RNFL reflectance, whereas the retinas with 2-mm and 3-mm ON remaining were optically stable. Fig. 1 shows the time courses of the reflectance measured on bundle and gap. Table 1 summarizes the changes of reflectance in each retina.
Axotomy too close to the globe causes acute changes in both the ganglion cell axons in the RNFL and the tissue of the surrounding retina, and should be avoided when using the ONT model to study pathophysiological mechanisms of ON injury.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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