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Tingting Jiang, Junxiang Gu, Qing Chang, Gezhi Xu; Resveratrol inhibits high glucose-induced metabolic memory of inflammation in HRMECs.. Invest. Ophthalmol. Vis. Sci. 2018;59(9):3091.
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Epidemiologic and prospective studies have shown that diabetic retinopathy（DR） continues beyond the point when good glycemic control has been achieved, and have suggested a "metabolic memory"phenomenon. Resveratrol (RSV), a potent SIRT1 activator, is a polyphenolic compound that is naturally produced by several plants. In recent years, numerous investigations have focused on the role of RSV in prevention or treatment of diabetes complications. However, little is known about the effect of resveratrol on diabetic retinopathy associated with metabolic memory phenomenon . Chronic inflammation has been implicated in the pathophysiology of DR. Therefore, the aim of the present study was to investigate the protective effects of RSV on the occurrence of “metabolic memory” of high glucose-induced inflammatory injury.
Human retinal microvascular endothelial cells (HRMECs) were cultured in either normal glucose /high glucose media for 8 days, or 4 days of high glucose followed by 4 days of normal glucose, with or without RSV treatment. Protein expressions of SIRT1,acetylated-nuclear factor κB (Ac-NF-κB),NLRP3 inflammasome,caspase-1 and IL-1β were examined by Western Blot. For multiple comparisons, one-way ANOVA followed by Tukey's multiple comparison tests were used. Differences were considered statistically significant when p-value was less than 0.05.
We found decreased protein expression of SIRT1 and increased expressions of Ac-NF-κB, NLRP3 inflammasome, caspase-1 and IL-1β in high glucose group compared to normal glucose group(P<0.05). Furthermore, these effects still persistent even after the return to normal glucose. Resveratrol (1 and 10 umol/l) treatment could enhance SIRT1 and protect against persistent upregulation of inflammatory factors(P<0.05).
Our data demonstrated that resveratrol administration relieved the persistent inflammatory insults induced by high glucose in HRECs. Therefore, it may serve as a promising therapeutic drug against the development of “metabolic memory" in diabetic retinopathy.
This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.
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