Rats were euthanized at 1, 3, 7, 14, and 28 days after injection. Eyes were enucleated and processed for either flatmount immunohistochemistry or cryopreserved (n = 3 at each age cryopreservation and flatmount). For flatmount histology, the retinas separated from the RPE/choroid complex. Tissues were fixed in 2% paraformaldehyde (PFA) in Tris-buffered saline (TBS) at 4°C overnight. The flatmount retinas were blocked in 5% goat serum (prepared in TBS with 0.1% Triton X-100 + 1% BSA) for 5 hours before incubation in primary antibodies overnight at 4°C. Retinas were incubated in chicken anti–glial fibrillary acidic protein (GFAP; activated Müller cells and astrocytes; 1:500; Millipore, Burlington, MA, USA) and rabbit anti-vimentin (Müller cells; 1:200, ab45939; Abcam, Inc., Cambridge, MA, USA), whereas RPE/choroid eyecups were stained with mouse anti-RPE65 (1:200; NB100-355, Novus Biologics, Littleton, CO, USA) and rabbit anti-CD34 (1:200; ab81289; Abcam, Inc.). After washes, tissues were incubated in secondary antibodies (goat anti-rabbit Alexa Fluor 647 [A21244; Invitrogen, Waltham, MA, USA], goat anti-chicken cyanine 3 [105-165-155; Jackson Immunoresearch, West Grove, PA, USA], and goat anti-mouse cyanine3 [115-165-003; Jackson Immunoresearch]) overnight. FITC-conjugated peanut agglutin lectin (PNA; 1:500; Sigma) was added to the secondary antibody cocktail for retinas. Flatmounts were imaged using Zeiss 510 or 710 Meta confocal microscopes equipped with Zen software (Carl Zeiss, Thornwood, NY, USA).