July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Outer Retinal Oxidative Stress Measured In Vivo using QUEnch-assiSTed (QUEST) OCT
Author Affiliations & Notes
  • Haohua Qian
    Visual Function Core, National Eye Institute, Bethesda, Maryland, United States
  • Robert H Podolsky
    Beaumont Research Institute, Michigan, United States
  • Karen M Lins-Childers
    Beaumont Research Institute, Michigan, United States
  • Yichao Li
    Visual Function Core, National Eye Institute, Bethesda, Maryland, United States
  • Bruce A Berkowitz
    Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, United States
  • Footnotes
    Commercial Relationships   Haohua Qian, None; Robert Podolsky, None; Karen Lins-Childers, None; Yichao Li, None; Bruce Berkowitz, None
  • Footnotes
    Support  EY026584, EY04068, AG058171, NEI intramural research program, an unrestricted grant from Research to Prevent Blindness (Kresge Eye Institute).
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 184. doi:
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      Haohua Qian, Robert H Podolsky, Karen M Lins-Childers, Yichao Li, Bruce A Berkowitz; Outer Retinal Oxidative Stress Measured In Vivo using QUEnch-assiSTed (QUEST) OCT. Invest. Ophthalmol. Vis. Sci. 2019;60(9):184.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To test the hypothesis that oxidative stress in the outer retina can be detected using antioxidants (AO’s) to correct an impaired light-evoked response as measured by OCT.

Methods : C57BL/6 mice were maintained in the dark for ~20 hours and studied by OCT before and after 1 hour of light exposure. Outer retina (OR, from outer limiting membrane to the retinal pigment epithelium–choroid boundary) thickness in dark or light were measured, and the light-dark difference (i.e., the photoresponse) was calculated. Subgroups of mice were given either saline or d-cis-diltiazem (an inducer of transient and non-damaging OR oxidative stress) ± AO's (methylene blue given 24 hours prior to examination and α-lipoic acid applied 1 hour prior to examination); one group was given only AO’s but kept in the dark.

Results : In uninjected or saline injected control mice, the OR showed a similar and reproducible light-induced expansion; AO’s given to dark adapted mice did not increase dark adapted OR thickness. d-cis-diltiazem treated mice had no photoresponse (p > 0.05). Administration of AO’s corrected (p < 0.05) the suppressed OR photoresponse, indicating the presence of oxidative stress in d-cis-diltiazem treated mice.

Conclusions : QUEnch-assiSTed (QUEST) OCT reproduced results from previous gold-standard assays showing that oxidative stress impairs the OR photoresponse, and that d-cis-diltiazem produces OR oxidative stress. We envision future applications of QUEST OCT in a range of oxidative stress-based retinopathies.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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