July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Quantitative analysis of retinal structure and function in two chromosomally altered mouse models of Down syndrome
Author Affiliations & Notes
  • Alberto Costa
    Case Western Reserve University School of Medicine, Cleveland, Ohio, United States
  • Daniella B Victorino
    Case Western Reserve University School of Medicine, Cleveland, Ohio, United States
    Postgraduate Program in Neurology and Neuroscience, Federal University of São Paulo, São Paulo, SP, Brazil
  • Jonah J Scott-McKean
    Case Western Reserve University School of Medicine, Cleveland, Ohio, United States
  • Footnotes
    Commercial Relationships   Alberto Costa, None; Daniella Victorino, None; Jonah Scott-McKean, None
  • Footnotes
    Support  This work was supported by ALANA USA Foundation (Contract #124124), The Hartwell Foundation Biomedical Research Fellowship, Awakening Angels. DBV is currently funded by postgraduate fellowship from São Paulo Research Foundation (FAPESP #2016/17746-3).
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 205. doi:
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      Alberto Costa, Daniella B Victorino, Jonah J Scott-McKean; Quantitative analysis of retinal structure and function in two chromosomally altered mouse models of Down syndrome. Invest. Ophthalmol. Vis. Sci. 2019;60(9):205.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Ophthalmic disorders are among the most prevalent comorbidities of Down syndrome (DS). Therefore, when studying mouse models of DS, ignoring how vision is affected in these animals can lead to misinterpretation of results from behavioral tests highly dependent on the integrity of the visual system. Here, we used optical imaging technologies and electroretinography (ERG) to study eye structure and function in two important mouse models of DS: Ts65Dn and Dp(16)1Yey/+.

Methods : Cornea and anterior segment were examined by slit-lamp. Thickness of retinal layers was quantified by Optical Coherence Tomography (OCT). Retinal vasculature parameters were assessed in vivo by bright field and fluorescent imaging, and in vitro by retinal flat-mount preparations. A Ganzfeld ERG system was used to assess retinal function in adult mice by evaluating ERG responses to flash stimuli of various intensities.

Results : Total retinal thickness is significantly increased in Ts65Dn and Dp(16)1Yey/+ compared with control mice; primarily due to increased thickness of the inner nuclear layer (INL). Additionally, increased retinal vessel caliber was found in chromosomally altered mice when compared with their respective controls. ERG responses in Ts65Dn and Dp(16)1Yey/+ mice showed subtle, but significant alterations compared to their controls. ERG properties were independent of the thickness of the INL, but dependent on the anesthetic agent used (ketamine, tribromoethanol, or urethane).

Conclusions : We provide evidence of retinal alterations in Ts65Dn and Dp(16)1Yey/+ mice that are similar to those reported in persons with DS. Our ERG results should raise caution about the choice of anesthetic agents in ERG experiments.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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