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Kyong Jin Cho, Jeongyun Kim, Zu Un Kim; Effect of parthenocissus tricuspidata extract living on pine in pre-clinical model of dry eye disease. Invest. Ophthalmol. Vis. Sci. 2019;60(9):264.
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Dry eye is a multifactorial disease of the ocular surface characterized by a loss of homeostasis of the tear film, and accompanied by ocular symptoms, in which tear film instability and hyperosmolarity, ocular surface inflammation and damage, and neurosensory abnormalities play etiological roles. Studies have shown that oxidative stress is also associated with dry eye disease, and drying of the ocular surface can lead to exposure to oxidative stress and worsen dry eye disease. Parthenocissus tricuspidata extract living on pine (PT) has been reported to exhibit anti-inflammatory activity as well as antioxidant activity. The purpose of this sdudy was to analyse the effect of parthenocissus tricuspidata extract living on pine in non-clinical model of dry eye disease.
In this study, we investigated the antioxidative and protective effects of PT on human conjunctival epithelial cells (WKD) after inducing oxidative stress with H2O2. WKD cells were seeded in 96-well culture plate at cell density of 8 × 103 cells/well and incubated for 24 hr before treatment. Cells were pre-treated with PT (50, 100, 500 μg/mL) for 1 hr and then exposed to 70 μM H2O2 for 24 hr. In addition, the anti-inflammatory and therapeutic effects of PT were also confirmed in the mouse model of dry eye disease. The dry eye disease mouse models were induced by subcutaneous injection of 2.5mg/mL scopolamine hydrobromide 4 times a day and exposure to desiccation stress including an air draft using a fan for 18 hr daily for 19 days.
PT treatment inhibited H2O2-induced apoptosis and reduced reactive oxygen species (ROS). In addition, PT inhibited the phosphorylation of the MAPK signaling pathway (ERK, JNK, p-38) as compared to the control group. ELISA showed antioxidative effects of SOD, CAT and MDA, and anti-inflammatory effect(decreased IL-6) in PT treatment group. In animal model of dry eye disease, tear volume and tear film break up time (TBUT) were increased in PT treatment(eye drop) group. In addition, the expression of inflammatory cytokines(IL-6, IL-1, TNF-α) were decreased.
Parthenocissus tricuspidata extract showed antioxidant, anti-inflammatoiry, and therapeutic effects in dry eye disease model (in vitro and in vivo), and it could be applied to the development and treatment of dry eye disease.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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