July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Effects of topical dry eye treatment on anxiety-related behavior in a mouse dry eye model
Author Affiliations & Notes
  • Takashi Kojima
    Ophthalmology, Keio Univ School of Medicine, Nagoya, AICHI, Japan
  • Sayaka Omura
    Ophthalmology, Keio Univ School of Medicine, Nagoya, AICHI, Japan
  • Taeko Nagata
    Ophthalmology, Keio Univ School of Medicine, Nagoya, AICHI, Japan
  • Cem Simsek
    Ophthalmology, Keio Univ School of Medicine, Nagoya, AICHI, Japan
  • Murat Dogru
    Ophthalmology, Keio Univ School of Medicine, Nagoya, AICHI, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio Univ School of Medicine, Nagoya, AICHI, Japan
  • Footnotes
    Commercial Relationships   Takashi Kojima, None; Sayaka Omura, None; Taeko Nagata, None; Cem Simsek, None; Murat Dogru, None; Kazuo Tsubota, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 289. doi:
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      Takashi Kojima, Sayaka Omura, Taeko Nagata, Cem Simsek, Murat Dogru, Kazuo Tsubota; Effects of topical dry eye treatment on anxiety-related behavior in a mouse dry eye model. Invest. Ophthalmol. Vis. Sci. 2019;60(9):289.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the effects of topical dry eye treatment on anxiety-related behavior in environmental dry eye stress (EDES) model mouse

Methods : Thirty C57BL/6 mice were divided into three treatment groups; vehicle group (artificial tear), HA group (0.1% hyaluronic acid) and rebamipide group (2% rebamipide). Mice were exposed to EDES for 3 days (5h/day). EDES mimicked a stressful office working environment. In each group, eye drops were initiated 5 days prior to EDES (4 times a day) and was continued during EDES (2 times a day). Before and after EDES, the following examinations were performed; aqueous tear secretion volume, vital stainings including fluorescein and lissamine green staining scores. Behavior tests including open-field test and elevated plus-maze test were conducted to analyze anxiety-related behavior. Two-way repeated measures ANOVA was performed using Graphpad Prism software. A p value less than 5% was considered to be a significant.

Results : In all three groups, the tear secretion volume significantly decreased (p<0.0001), and the vital staining scores significantly increased after EDES exposure (p<0.0001). After exposure to EDES, the mean tear secretion quantity in the rebamipide group (0.16±0.03mm/g) showed a significantly higher value than the vehicle (0.11±0.04 mm/g, p=0.004) and the HA group (0.12±0.05mm/g, p=0.016). Similarly, the mean fluorescein staining score in the rebamipide group (4.3±0.67pts) was significantly lower than the vehicle (3.1±0.74pts, p=0.03). The mean lissamine green staining score in the rebamipide group (3.3±0.68 pts) was significantly lower than the vehicle (4.2±0.92pts, p=0.016) and the HA group (4.0±0.67pts, p=0.003).
Center staying time in open-field test decreased significantly after EDES exposure in all groups (p<0.0001). There were no significant differences between treatment groups (p=0.113). Open arms staying time in the elevated plus-maze test in the rebamipide group (53.7±12.0s) was significantly longer than the vehicle (27.0±19.6s, p=0.007) and HA group (31.0±15.6s, p=0.011).

Conclusions : Our current research found that topical dry eye treatment could affect anxiety-related behavior in dry eye model mice. A dry eye remedy with a superior treatment effect was associated with lesser induction of anxiety-related behavior.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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