July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Analysis of the tear MicroRNA levels in primary sjögren’s syndrome
Author Affiliations & Notes
  • Yu Jeong Kim
    Hanyang University Hospital, Seoul, Korea (the Republic of)
  • Yeji Yeon
    Hanyang University Hospital, Seoul, Korea (the Republic of)
  • Jaeyoung Kim
    Seoul Paik Hospital, Korea (the Republic of)
  • Yong Un Shin
    Hanyang University Hospital, Seoul, Korea (the Republic of)
  • Heeyoon Cho
    Hanyang University Hospital, Seoul, Korea (the Republic of)
  • Han Woong Lim
    Hanyang University Hospital, Seoul, Korea (the Republic of)
  • Min Ho Kang
    Hanyang University Hospital, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Yu Jeong Kim, None; Yeji Yeon, None; Jaeyoung Kim, None; Yong Un Shin, None; Heeyoon Cho, None; Han Woong Lim, None; Min Ho Kang, None
  • Footnotes
    Support  National Research Foundation of Korea (2017R1D1A1B03034507)
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 297. doi:
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      Yu Jeong Kim, Yeji Yeon, Jaeyoung Kim, Yong Un Shin, Heeyoon Cho, Han Woong Lim, Min Ho Kang; Analysis of the tear MicroRNA levels in primary sjögren’s syndrome. Invest. Ophthalmol. Vis. Sci. 2019;60(9):297.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We investigated the expression of microRNAs (miRNAs) in the tears of patients with primary sjögren’s syndrome (pSS) compared to the tears of healthy controls. And we also examined the correlation between miRNAs expression and ocular parameters.

Methods : Eighteen tear samples were collected from pSS patients and eight from normal subjects at the Hanyang University Hospital. Clinical ophthalmologic assessments included Schirmer I test, tear film breakup time (tBUT) and ocular staining score (OSS). The expression of 43 different miRNAs were measured by real-time polymerase chain reaction. Differentially expressed miRNAs were stratified for fold regulation (FR) larger than ±2 and for significance of P < 0.05.

Results : For this study, 18 patients with primary SS (mean age 47.22 ± 11.56 years) and 8 controls (mean age 42.5 ± 11.78 years) were included. We found four miRNAs with significantly different expression in pSS patients compared to controls. Expression levels of miR-16-5p (FR=2.34, P=0.009) in patients with SS was significantly higher than that in controls. Expression levels of miR-30b-5p (FR=−2.14, P=0.023), miR-30c-5p (FR=−3.50, P=0.043), miR-203a-3p (FR=−2.28, P=0.031) in patients with primary SS were significantly lower than that in controls. All four miRNAs were not significantly correlated with OSS scores.

Conclusions : Our findings indicate that miR-16-5p, miR-30c-5p, miR-203a-3p, miR-30b-5p are deregulated in pSS. These miRNAs may play a role in the pathogenesis of pSS and serve as biomarkers for diagnosis of pSS.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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