July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Gene expression in the corneal epithelium of progressive keratoconus patients
Author Affiliations & Notes
  • Uri Soiberman
    Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States
  • Footnotes
    Commercial Relationships   Uri Soiberman, None
  • Footnotes
    Support  NEI K08EY027474
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 333. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Uri Soiberman; Gene expression in the corneal epithelium of progressive keratoconus patients. Invest. Ophthalmol. Vis. Sci. 2019;60(9):333.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose : To compare the corneal epithelial gene expression profile of progressive keratoconus (KC) patients and healthy myopic patients.

Methods : Genome-wide transcriptome analyses by RNA-sequencing from total RNA isolated from the epithelial tissue of individual cases of progressive KC undergoing epithelium-off corneal collagen cross-linking. The control group consisted of healthy myopic controls undergoing myopic photorefractive keratectomy (PRK). The latter group was screened to rule out KC prior to PRK. These results were then verified using TaqMan PCR performed on the initial cohort and additional samples.

Results : Differential gene expression was found in samples originating from patients with progressive KC. Specifically, WNT10A was found to be underexpressed in KC (relative expression: 0.6, p=0.03) as was PRUNE2 (0.62, p=0.03), whereas NRAPR1 was overexpressed (1.84, p=0.02).

Conclusions : The differential gene expression in the corneal epithelium of progressive KC suggests that the abnormalities observed in KC corneas are not limited to the stroma, and that pathological process may also involve the corneal epithelium.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.


This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.