July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
In-vivo and in-vitro corneal epithelial and stromal thickness in Keratoconus
Nicole Hallett; Vinod Kumar Maseedupally; Maria Markoulli; Niroshan Jeyakumar; Christopher Hodge; Gerard Sutton; Jingjing You
Author Affiliations & Notes
  • Nicole Hallett
    Discipline of Opthalmology, Sydney Medical School , Sydney University, Save Sight Institute, Collaroy, New South Wales, Australia
    School of Optometry and Vision Sciences, University of New South Wales, Sydney, New South Wales, Australia
  • Vinod Kumar Maseedupally
    School of Optometry and Vision Sciences, University of New South Wales, Sydney, New South Wales, Australia
  • Maria Markoulli
    School of Optometry and Vision Sciences, University of New South Wales, Sydney, New South Wales, Australia
  • Niroshan Jeyakumar
    School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia
  • Christopher Hodge
    Discipline of Opthalmology, Sydney Medical School , Sydney University, Save Sight Institute, Collaroy, New South Wales, Australia
    Vision Eye Institute, Chatswood, New South Wales, Australia
  • Gerard Sutton
    Discipline of Opthalmology, Sydney Medical School , Sydney University, Save Sight Institute, Collaroy, New South Wales, Australia
    NSW Tissue Bank, Sydney, New South Wales, Australia
  • Jingjing You
    Discipline of Opthalmology, Sydney Medical School , Sydney University, Save Sight Institute, Collaroy, New South Wales, Australia
    NSW Tissue Bank, Sydney, New South Wales, Australia
  • Footnotes
    Commercial Relationships   Nicole Hallett, None; Vinod Maseedupally, None; Maria Markoulli, None; Niroshan Jeyakumar, None; Christopher Hodge, None; Gerard Sutton, None; Jingjing You, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 334. doi:
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      Nicole Hallett, Vinod Kumar Maseedupally, Maria Markoulli, Niroshan Jeyakumar, Christopher Hodge, Gerard Sutton, Jingjing You; In-vivo and in-vitro corneal epithelial and stromal thickness in Keratoconus. Invest. Ophthalmol. Vis. Sci. 2019;60(9):334.

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Abstract

Purpose : To compare epithelial and stromal thicknesses and their relationship between Keratoconus (KC) and control eyes in vivo and in vitro.

Methods : Epithelial and stromal thickness was retrospectively measured [HC17316] using 3D OCT (CASIA Tomey SS 1000) on 8 participants with KC (aged 33±14.2 yrs) and 23 controls: 12 emmetropic (24.4±5.9 yrs) and 11 myopic (21.5±1.9 yrs). Thickness (µm) was manually delineated at 0.5mm intervals along the horizontal corneal meridian from the central apex, to a chord width of 10mm. The thinnest corneal region was obtained by determining the average clustered thinnest region. In vitro measurement of corneal epithelial and stromal thickness was measured on 22 haematoxylin and eosin stained corneal sections (13KC, 9 controls) microscopically by Image J. Normality was assessed with Shapiro-Wilk for normal data and Kruskal-Wallis for non-normal data, analysis of variance was performed with a significance level at p = 0.05. Study appoved by local HREC (2013-1041).

Results : In vivo, significant differences were found in mean epithelial thickness in KC eyes (45.3±2.2µm) compared to both myopic (51.4±3.1µm, p <0.05) and emmetropic controls (52.9±1.7µm, p<0.05). Mean stromal thickness was significantly different only in the thinnest region of cornea between KC eyes (452.0±36.2µm) compared to both myopic (536.4±22.8µm, p<0.05) and emmetropic controls (531.8±32.7µm, p<0.05). The ratio between the epithelium and the stroma was significantly different only in KC eyes (0.09±0.008µm) compared to emmetropic controls (0.99±.05µm, p=0.02). There was no relationship between epithelial and stromal thickness in both the myopic (r2=0.20, p=0.15) and emmetropic control groups (r2=0.15, p=0.207) or in the KC group (r2=0.80 p=0.40). In vitro, mean epithelial thickness was significantly higher in KC eyes (49.2±3.3µm) compared to controls (33.6±2.9µm, p=0.003).

Conclusions : In-vivo epithelial thickness is significantly reduced in KC compared to myopic and emmetropic eyes, and greater thickness changes were observed in epithelium compared to stroma in KC. Epithelial and stromal thinning in the KC thinnest region was observed, indicating a doughnut shape of significantly thicker epithelium in the KC adjacent area. In vitro the reverse was found where epithelium was higher in KC eyes. This suggests that in-vitro specimens and in-vivo measures may present different representations of KC or result from different stages.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright © 2024 Association for Research in Vision and Ophthalmology.
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