Abstract
Purpose :
Implantation of an intraocular lens was the standard of care to correct the aphakic state following the removal of cataract. Ideally, the IOL was placed in the capsular bag. While capsular reconstruction was determined by complex factors (the state of capsule, iris). The research was to evaluate the relationship between the cytokine profile in aqueous humor and the state of capsule after Lensectomy and Anterior Vitrectomy.
Methods :
We followed a cohort of 58 eyes with congenital cataract from Wenzhou medical university, 26 eyes without cataract surgery and 33 eyes with congenital cataract was after lensectomy and anterior vitrectomy. Aqueous humor (100–200 μl) was aspirated from each patient by means of limbic paracentesis with the use of 30 gauge needle before the surgery. The procedure was performed under a surgical microscope. The samples were snap frozen and maintained at −80°C until use. Levels of various inflammation-related cytokines in the aqueous humor of using multiplex immunoassay.
Results :
The mean concentration of IL-6 (6.64±12.57pg/mL) was significantly higher in eyes after surgery compared to the controls (2.25±5.87pg/mL; P =0.000) and the mean concentration of IL-2 (50.68±21.50 pg/mL) was significantly higher in eyes after surgery than in the controls (42.63±13.83pg/mL; P=0.012). Furthermore, the IP-10, IL-10 and IL-2 were also higher in in eyes after surgery. There were no differences in the concentration of TGF-beta 2, TNF-α, IL-1β, PDGFBB, IFNγ, IL1α, MIP1α, MIP1β, IL-4, IL-17a, IL-5, Eotaxin. Furthermore, we divided the 33 eyes after surgery into three groups based on the shrinkage level of capsular bag (0=no constriction; 1=slight constriction; 2= severe constriction ;) The concentration of IL-6 was higher in capsular bag with severe constriction. While the aqueous humor was no significant changes in synechia iridis eyes.
Conclusions :
Higher preoperative levels of IL-6, IL-2 in aqueous humor were associated with capsular constriction, which indicated the inflammation state after lensectomy and anterior vitrectomy may affect the reconstruction of capsular bag.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.