July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Optogenetic restoration of vision in the macaque
Author Affiliations & Notes
  • William H Merigan
    Flaum Eye Institute, University of Rochester, Rochester, New York, United States
    Center for Visual Science, University of Rochester, Rochester, New York, United States
  • Footnotes
    Commercial Relationships   William Merigan, None
  • Footnotes
    Support  NIH grant R01EY021166
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 6. doi:
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      William H Merigan; Optogenetic restoration of vision in the macaque. Invest. Ophthalmol. Vis. Sci. 2019;60(9):6.

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      © ARVO (1962-2015); The Authors (2016-present)

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Presentation Description : We have recently restored retinal ganglion cell responses in the living macaque by inserting ChrimsonR into ganglion cells. This was accomplished in eyes in which visually-mediated ganglion cell responses were eliminated by a focal laser lesion of foveal photoreceptors. ChrimsonR was inserted into ganglion cells with a viral vector that targeted inner retina neurons. In addition, the calcium indicator G-CaMP6 was inserted into the same ganglion cells to permit in-vivo measurement of ganglion cell responses. Observation of the visually-driven responses by the fluorescent response of the calcium indicator required use of adaptive optics in vivo imaging. This is the first in vivo demonstration of optogenetically restored visual responses following damage to outer retina neurons.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.


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