July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Comparative anatomy and connectivity of the Aii amacrine cell in mouse and rabbit retina
Author Affiliations & Notes
  • Selena Wirthlin
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Crystal Sigulinsky
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • James Anderson
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Daniel Emrich
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Christopher Rapp
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Jeebika Dahal
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Rebecca L Pfeiffer
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Kevin Rapp
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Jia-Hui Yang
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Carl Watt
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Robert Marc
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Bryan W Jones
    Ophthamology & Visual Sciences, University of Utah, Salt Lake, Utah, United States
  • Footnotes
    Commercial Relationships   Selena Wirthlin, None; Crystal Sigulinsky, None; James Anderson, None; Daniel Emrich, None; Christopher Rapp, None; Jeebika Dahal, None; Rebecca Pfeiffer, None; Kevin Rapp, None; Jia-Hui Yang, None; Carl Watt, None; Robert Marc, Signature Immunologies Inc. (I); Bryan Jones, None
  • Footnotes
    Support  R01 Grant EY02576, T32 Grant EY024234, P30 Grant EY014800, Undergraduate research awards from the Undergraduate Research Opportunities Program at the University of Utah, Unrestricted Grant from Research to Prevent Blindness to the Department of Ophthalmology & Visual Sciences
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 548. doi:https://doi.org/
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      Selena Wirthlin, Crystal Sigulinsky, James Anderson, Daniel Emrich, Christopher Rapp, Jeebika Dahal, Rebecca L Pfeiffer, Kevin Rapp, Jia-Hui Yang, Carl Watt, Robert Marc, Bryan W Jones; Comparative anatomy and connectivity of the Aii amacrine cell in mouse and rabbit retina. Invest. Ophthalmol. Vis. Sci. 2019;60(9):548. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Mouse retina structurally differs from rabbit retina, as it is thicker and vascularized, while the rabbit retina is thinner and avascular. The implications of these differences on neuronal morphology and connectivity is not known. This project compares the morphology and connectivity of the Aii amacrine cell (AC) with ultrastructural precision in connectomes of mouse (RC2) and rabbit (RC1) retina.

Methods : RC1 and RC2 are connectomes built by automated transmission electron microscopy at ultrastructural (2 nm/pixel) resolution. RC1 and RC2 are 0.25mm diameter volumes of retina. RC1 is from a 13 month old, female Dutch Belted rabbit. RC2 is from a 5 month old female C57BL/6J mouse. The Viking application was used to annotate Aii ACs in both connectomes.

Results : Mouse Aii ACs are noticeably elongated to span the thicker inner plexiform layer (IPL) and have a prominent neck region. Lobular appendages of Aii ACs in both species extend thin stalks from the soma, neck and proximal arboreal dendrites in the OFF sublamina, predominantly forming reciprocal synapses with OFF cone bipolar cells (BCs). In rabbits, multiple arboreal dendrites emerge from the base of the neck, branch and travel obliquely through the ON sublamina, and form gap junctions with ON cone BCs, neighbor Aii ACs, and itself. They extend laterally at the base of the IPL, collecting ribbon input from rod BCs. In contrast, mouse arboreal dendrites stem from a single primary dendrite that branches as it travels vertically through the IPL without self-branch interaction, terminating at variable depths that align with the more broadly ramified axon terminals of rod BCs. Conventional synapse to gap junction ratios reveal greater output in the OFF vs ON layer in mouse compared to rabbit. Notably, mouse Aii ACs form gap junctions with the descending axons of ON cone BCs as they pass its soma, in contrast to rabbit, where gap junctions do not form at contacts proximal to ON cone BC axon terminals.

Conclusions : Lateral expansion of rabbit Aii ACs may be attributable to eccentricity. However, morphological differences appear to mediate greater output to the OFF versus ON pathway in mouse. Synaptic partners are currently being analyzed. Comparative anatomy connectomics is essential for understanding possible implications of retinal structure on neuronal morphology and connectivity that may underlie network differences between the mouse and rabbit retina.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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