July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Vitreal cytokine profile in rats innoculated with bovine insoluble Melanin Associated Antigen, type 1 collagen, and type 2 collagen, and the influence of age and sex on uveitis
Author Affiliations & Notes
  • Stephanie Osinchuk
    Small Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
  • Tracy Wilson-Gerwing
    Pediatrics, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
  • Alan Rosenberg
    Pediatrics, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
  • Bruce Grahn
    Small Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
  • Footnotes
    Commercial Relationships   Stephanie Osinchuk, None; Tracy Wilson-Gerwing, None; Alan Rosenberg, None; Bruce Grahn, None
  • Footnotes
    Support  Children's Hospital Foundation of Saskatchewan
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 798. doi:
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      Stephanie Osinchuk, Tracy Wilson-Gerwing, Alan Rosenberg, Bruce Grahn; Vitreal cytokine profile in rats innoculated with bovine insoluble Melanin Associated Antigen, type 1 collagen, and type 2 collagen, and the influence of age and sex on uveitis. Invest. Ophthalmol. Vis. Sci. 2019;60(9):798.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine if the age and sex of rats at the time of innoculation influences the uveitogenicity of insoluble Melanin Associated Antigen (MAA), type I collagen, or type II collagen and to determine the vitreal cytokine profile in each treatment group.

Methods : Adult and juvenile male and female Lewis rats were inoculated intradermally with bovine type I collagen, type II collagen, or insoluble MAA. Biomicroscopic ophthalmic examinations were completed in live rats at baseline and repeated three time per week throughout the study period. At the termination of the experiment eyes were collected and vitreous sampled. Hematoxylin and eosin stained globes were evaluated using light microscopy. An array of 27 vitreal cytokines was analyzed using multiplex bead-based immune assay.

Results : Rats immunized with one injection of insoluble MAA had increased clinical uveitis scores, and uveitis on histopathology. There was no significant difference in disease incidence, or onset in uveitis between either age or sex. The insoluble MAA inoculated rats had significantly increased vitreal levels of pro-inflammatory cytokines RANTES and IP-10 when compared to control rats. Rats inoculated with either type I or type II collagen did not develop uveitis clinically nor histopathologically, and did not have cytokine values that differed from controls.

Conclusions : Inoculation with insoluble MAA results in elevations in vitreal RANTES and IP-10. No significant difference in uveitis incidence or onset was observed between sexes or age cohorts.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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