July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
 

Comparison of cytokine concentrations in vitreous fluids among uveitis diseases

Author Affiliations & Notes
  • Toshikatsu Kaburaki
    Ophthalmology, Univ of Tokyo School of Medicine, Bunkyo-ku, Tokyo, Japan
  • Hisako Ono
    Ophthalmology, Univ of Tokyo School of Medicine, Bunkyo-ku, Tokyo, Japan
  • Rie Tanaka
    Ophthalmology, Univ of Tokyo School of Medicine, Bunkyo-ku, Tokyo, Japan
  • Tomohito Sato
    Ophthalmology, National Defense Medical College Hospital, Tokorozawa, Saitama, Japan
  • Masaru Takeuchi
    Ophthalmology, National Defense Medical College Hospital, Tokorozawa, Saitama, Japan
  • Hideto Tozawa
    Isotope Science Center, Bunkyo-ku, Tokyo, Japan
  • Mari Katsura
    Isotope Science Center, Bunkyo-ku, Tokyo, Japan
  • Wada Youichiro
    Isotope Science Center, Bunkyo-ku, Tokyo, Japan
  • Shintaro Shirahama
    Ophthalmology, Univ of Tokyo School of Medicine, Bunkyo-ku, Tokyo, Japan
  • Hirotsugu Soga
    Ophthalmology, Univ of Tokyo School of Medicine, Bunkyo-ku, Tokyo, Japan
  • Hidetoshi Kawashima
    Ophthalmology, Jichi Medical University, Shimotsuke, Tochigi, Japan
  • Makoto Aihara
    Ophthalmology, Univ of Tokyo School of Medicine, Bunkyo-ku, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Toshikatsu Kaburaki, Alcon and Novartis Pharma (F), Eisai (F); Hisako Ono, None; Rie Tanaka, None; Tomohito Sato, None; Masaru Takeuchi, None; Hideto Tozawa, None; Mari Katsura, None; Wada Youichiro, None; Shintaro Shirahama, None; Hirotsugu Soga, None; Hidetoshi Kawashima, None; Makoto Aihara, None
  • Footnotes
    Support  Japan Society for Promotion of Science (18K09398), Japan Health Sciences Research from MHLW (H29-nanti-ippan-050), Grant-in-Aid for Eisai Japan (2018), Alcon and Novartis Pharma (2018)
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 812. doi:https://doi.org/
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    • Get Citation

      Toshikatsu Kaburaki, Hisako Ono, Rie Tanaka, Tomohito Sato, Masaru Takeuchi, Hideto Tozawa, Mari Katsura, Wada Youichiro, Shintaro Shirahama, Hirotsugu Soga, Hidetoshi Kawashima, Makoto Aihara;  

      Comparison of cytokine concentrations in vitreous fluids among uveitis diseases

      . Invest. Ophthalmol. Vis. Sci. 2019;60(9):812. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To compare cytokine concentrations in vitreous fluids (VF) among representative uveitis diseases.

Methods : A total of 63 uveitis patients (sarcoidosis: 16 cases, acute retinal necrosis (ARN): 16 cases, bacterial endophthalmitis: 16 cases, intraocular malignant lymphoma (IOL): 15 cases) and 15 patients with idiopathic epiretinal membrane (ERM, 15 cases) who had received pars plana vitrectomy were enrolled in this study. Vitreous samples (100 µL) were collected at the time of surgery and cryopreserved. The concentrations of 34 inflammatory cytokines and chemokines were examined using Bio-Plex Multiplex Immunoassays (BioRad), and the characteristics of the elevated cytokines in each disease were examined.

Results : Most of inflammatory cytokines were elevated in VF in uveitis patients compared to those in patients with ERM. Especially, proinflammatory cytokines (Interleukin-1 receptor antagonist (IL-1RA), Interleukin (IL)-2, IL-5, IL-6, Tumor necrosis factor (TNF)-α, Interferon (IFN)-γ) and chemokines (Macrophage inflammatory protein (MIP)-1α, MIP- 1β, Regulated on activation normal T cell expressed and secreted (RANTES)) were much higher in VF of uveitis patients compared to those in ERM patients. On the other hand, compared to VF with sarcoidosis, IL-1RA, IL-6, IL-10, Granulocyte colony-stimulating Factor (G-CSF), MIP-1α, MIP-1β and Vascular Endothelial Growth Factor (VEGF) were significantly higher in VF with ARN and bacterial endophthalmitis. Moreover, IFN-α2 in ARN, G-CSF in bacterial endophthalmitis and IL-10 in IOL were much higher than those of the other types of uveitis.

Conclusions : Elevated patterns of cytokine concentrations in VF indicated a characteristic manner according to causative diseases of uveitis. They might be involved in the pathogenesis of each uveitis.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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