July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Sirtuin1 deficiency suppresses migration during corneal epithelial wound healing
Author Affiliations & Notes
  • yong lin
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Li Li
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Qi Liu
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Dongsheng Yan
    School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China
    State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou, Zhejiang, China
  • Footnotes
    Commercial Relationships   yong lin, None; Li Li, None; Qi Liu, None; Dongsheng Yan, None
  • Footnotes
    Support  973 Projects (2012CB722303&2011CB504605) from the Ministry of Science and Technology of China.
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 923. doi:https://doi.org/
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    • Get Citation

      yong lin, Li Li, Qi Liu, Dongsheng Yan; Sirtuin1 deficiency suppresses migration during corneal epithelial wound healing. Invest. Ophthalmol. Vis. Sci. 2019;60(9):923. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Sirtuin1 (SIRT1), a nicotinamide adenine dinucleotide (NAD+) dependent deacetylase, has been demonstrated to play critical roles in various biological processes. However, the precise role of SIRT1 in corneal epithelium (CE) is still poorly understood. Here, we investigated its function during corneal epithelial wound healing (CEWH).

Methods : Mouse CEWH model was created by CE debridement using Algerbrush. Realtime RT-PCR and Western blot detected the expression level of SIRT1 during CEWH. CE-specific Sirt1 deletion mice were generated using Cre-lox system. SIRT1 was knocked down in human corneal epithelial cells (HCECs) using Lipofectamine-mediated siRNA transfection. HCEC proliferation and migration was examined by MTS and scratch assay, respectively.

Results : SIRT1 was significantly upregulated during CEWH. Sirt1 deletion markedly delayed in vivo CEWH. Moreover, downregulation of SIRT1 dramatically inhibited HCEC migration.

Conclusions : Our results demonstrate that SIRT1 is critical in regulating CEWH, which implys an effective therapeutic strategy to enhance CEWH.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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