Abstract
Purpose :
To investigate the effects of Conbercept on rabbit corneal neovascularization (CNV) after alkali burn.
Methods :
Corneal neovascularization was established by alkali burn in 15 rabbits (15 eyes). The rabbits were divided into 3 groups randomly. The experimental group, the control group and the blank group (n=5) was given intramuscular injection of Conbercept (10mg/ml,0.05ml) in the corneal stroma 2 weeks after modeling. The control group was given equal amount of PBS at the same time. The blank group was given no treatment. The corneal neovascularization was observed by the slit lamp and the rabbit cornea was photographed 2/3/4/5/6 weeks after alkali burn. The CNV area was calculated. Three rats were randomly selected from the three group for 2/3/4/5/6 weeks after alkali burn, the aqueous humor was obtained and enzyme-linked immunoassay (ELISA) was used to detect the content of VEGF; Immunofluorescence was used to observe the expression of vascular endothelial growth factor. The statistical analysis was performed using SPSS19.0 software.
Results :
The area of corneal neovascularization was smaller in all experimental group than in the control group and the blank group (p<0.05). The concentration of VEGF in aqueous humor as significantly lower in the experimental group compared to the control and blank group (p<0.05).
Conclusions :
Conbercept can inhibit CNV in alkali burned rabbits.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.