July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Signals from sphingosine-1-phosphate receptor type 3 involvement in vascular formation by endothelium and in VEGF expression in macrophages in vitro.
Author Affiliations & Notes
  • Shingo Yasuda
    Wakayama Medical University, Wakayama city, Japan
  • Takayoshi Sumioka
    Wakayama Medical University, Wakayama city, Japan
  • Yuka Okada
    Wakayama Medical University, Wakayama city, Japan
  • Masayasu Miyajima
    Wakayama Medical University, Wakayama city, Japan
  • Kana Ichikawa
    Wakayama Medical University, Wakayama city, Japan
  • Shizuya Saika
    Wakayama Medical University, Wakayama city, Japan
  • Footnotes
    Commercial Relationships   Shingo Yasuda, None; Takayoshi Sumioka, None; Yuka Okada, None; Masayasu Miyajima, None; Kana Ichikawa, None; Shizuya Saika, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 948. doi:https://doi.org/
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      Shingo Yasuda, Takayoshi Sumioka, Yuka Okada, Masayasu Miyajima, Kana Ichikawa, Shizuya Saika; Signals from sphingosine-1-phosphate receptor type 3 involvement in vascular formation by endothelium and in VEGF expression in macrophages in vitro.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):948. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We here examined here roles of signals derived from sphingosine-1-phosphate (S1P) or its receptor type 3 (S1PR3) on neovascularization activity of human retinal microvascular endothelial cells (HRMECs) and on angiogenic growth factor expression in macrophages. Purpose was to clear the mechanism of suppression of corneal neovascularization in a mouse that lacks sphingosine-1-phosphate receptor type 3 (S1PR3) (reported by us in ARVO 2018) by using cell culture.

Methods : (1) HRMECs were cultured on matrigel and allowed to form vessel-like structures in three dimension culture. The degree of the formation of the structure was compared in the three groups (S1P+ group, S1P+ and S1PR3 inhibitor+ group, and control group) (2) Peritoneal macrophages were harvested 4 days after i. p. oyster glycogen (5 %, sterilized) in wild-type or S1PR3-null mice. mRNA expression levels of VEGF-A in macrophages were compared and examined using real-time RT-PCR.

Results : (1) Formation of vessel-like structute by HRMECs was promoted by S1P addition, and inhibited by further addition of S1PR3 inhibitor concentration-dependently. (2) The expression level of VEGF-A in macrophages was significantly suppressed by the loss of S1PR3.

Conclusions : The mechanisms of inhibition of neovascularization in the absence of S1PR3, reported in ARVO 2018, include both impairment of vascular structure formation by endothelial cells and reduction of VEGF-A in macrophages.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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