July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Impaired angiogenic response in cornea by lacking TRPV4 in mice
Author Affiliations & Notes
  • Takayoshi Sumioka
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Yuka Okada
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • HIroki Iwanishi
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Shingo Yasuda
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Masayasu Miyajima
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Shizuya Saika
    Ophthalmology, Wakayama Medical University, Wakayama, Wakayama, Japan
  • Footnotes
    Commercial Relationships   Takayoshi Sumioka, None; Yuka Okada, None; HIroki Iwanishi, None; Shingo Yasuda, None; Masayasu Miyajima, None; Shizuya Saika, None
  • Footnotes
    Support  Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research(C)
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 949. doi:https://doi.org/
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    • Get Citation

      Takayoshi Sumioka, Yuka Okada, HIroki Iwanishi, Shingo Yasuda, Masayasu Miyajima, Shizuya Saika; Impaired angiogenic response in cornea by lacking TRPV4 in mice. Invest. Ophthalmol. Vis. Sci. 2019;60(9):949. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the effects of loss of transient receptor potential vanilloid 4 (TRPV4) in the development of neovascularization in a corneal stroma in mice. TRPV4 is a cation channel type receptor involved in febrile hyperalgesia and pain as well as multiple cell behavior modulation as a mechanosensor.

Methods : (1) Corneal neovascularization from the limbal vessels was induced by cauterization of the central cornea of an eye of both C57BL/6 (WT) mice (n = 24) and TRPV4-null (KO) mice (n = 25) by a disposable tool of Accu-temp. The eye was processed for cryosectioning and were examined by using immunohistochemistry. Expression of angiogenic growth factors and inflammatory cell markers were examined in RNA samples derived from day 3 tissues and uninjured ones by using TaqMan real time-RT-PCR. (2) We employed in vitro assay of angiogenic activity of human umbilical vein endothelial cell (HUVEC). The culture was maintained in the routine culture condition in the presence of VEGF-A as an angiogenesis inducer in the presence or absence of a TRPV4 antagonist (HC-067047). The length of tube-like structure formation was compared and examined after 12 hours.

Results : (1) The length of the neovascularization from the limbus was less in KO mice as compared with WT mice at day 7 as revealed by CD31 immunostaining. Expression of mRNAs of F4/80 macrophage antigen was significantly less in a KO cornea as compared with a WT cornea at day 3. (2) The angiogenic activity in HUVEC was significantly suppressed by the addition of TRPV4 antagonist in a concentration-dependent manner.

Conclusions : TRPV4 signal is involved in macrophage infiltration and angiogenesis process in a mouse cornea.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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