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Kasumi Kikuchi, Kousuke Noda, Miyuki Murata, Yoshiaki Tagawa, Atsuhiro Kanda, Satoru Kase, Yasushi Kageyama, Masami Shinohara, Tomohiko Sasase, Susumu Ishida; Diabetic Cataract in Spontaneously Diabetic Torii Fatty Rats.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1123.
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© ARVO (1962-2015); The Authors (2016-present)
Spontaneously Diabetic Torii (SDT) fatty rat is a novel animal model of obese type 2 diabetes. Previously, it was shown that SDT fatty rats exhibited cataract formation as one of the diabetic ocular complications. However, time course of cataract development in SDT fatty rats has not been reported and its biochemical characteristics of the lens remain elusive. The aim of this study was to further investigate cataractogenesis in SDT fatty rats.
Male SDT fatty rats and age-matched SD rats (CLEA Japan, Inc.) were used. Body weight and blood glucose level were measured every week from 5 to 16 weeks of age. The time-course change of lens opacification was evaluated in 5 stages (stage 0: no cataract to 4: mature cataract) by a slit lamp examination. At 16 weeks of age, glucose and glutathione (GSH) concentrations were measured in the lens proteins.
At 16 weeks of age, mean body weight of SDT fatty rats (n=13) and SD rats (n=11) were 526±9.8g and 485±3.8g, respectively (p<0.01). Blood glucose levels in SDT fatty rats (769±21mg/dl) were significantly higher than those in SD rats (108±4mg/dl, p<0.01). At 5 weeks of age, none of rats showed cataract formation; however, 12 SDT fatty rats (92.3%) developed mature cataract (stage 4) and the others (7.7%) showed severe cataract (stage 3) at 16 weeks of age. The lens GSH concentration was lower in SDT fatty rats (7.1±0.4nmol/mg) than SD rats (16.7±0.4nmol/mg, p<0.01), and the level of glucose was higher in the lens of SDT fatty rats (11.3±0.5nmol/mg) than that of SD rats (0.2±0.1nmol/mg, p<0.01).
The present data demonstrated that cataractogenesis in SDT fatty rats was consistent with that in patients with diabetes, indicating that SDT fatty rats have the potential to serve as an animal model of diabetic cataract associated with type 2 diabetes and obesity.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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