July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Diabetic Cataract in Spontaneously Diabetic Torii Fatty Rats.
Author Affiliations & Notes
  • Kasumi Kikuchi
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • Kousuke Noda
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • Miyuki Murata
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • Yoshiaki Tagawa
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • Atsuhiro Kanda
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • Satoru Kase
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • Yasushi Kageyama
    CLEA Japan Inc., Tokyo Animal & Diet Dept., Tokyo, Japan
  • Masami Shinohara
    CLEA Japan Inc., Tokyo Animal & Diet Dept., Tokyo, Japan
  • Tomohiko Sasase
    Biological/Pharmacological Research Laboratories, Central Pharmaceutical Research Institute, Japan Tobacco Inc., Osaka, Japan
  • Susumu Ishida
    Laboratory of Ocular Cell Biology and Visual Science, Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan
  • Footnotes
    Commercial Relationships   Kasumi Kikuchi, None; Kousuke Noda, Alcon Pharma Co. Ltd. (F), Coopervision Inc. (F), Mitsubishi Tanabe Pharma Corp. (F), NIDEK Co., Ltd. (P), Novartis Pharma, K.K. (F), Otsuka Pharmaceutical Co., Ltd. (F), SEED Co., Ltd. (P), Senju Pharmaceutical Co., Ltd. (P), Sucampo Pharma, LLC. (F); Miyuki Murata, None; Yoshiaki Tagawa, None; Atsuhiro Kanda, Alcon Pharma Co. Ltd. (F), Bayer Yakuhin, Ltd. (F), Bonac Co., Ltd. (P), Novartis Pharma, K.K. (F), SEED Co., Ltd. (P), Senju Pharmaceutical Co., Ltd. (P); Satoru Kase, ROHTO Pharmaceutical Co., Ltd. (F); Yasushi Kageyama, CLEA Japan Inc. (E); Masami Shinohara, CLEA Japan Inc. (E); Tomohiko Sasase, Japan Tobacco Inc. (E); Susumu Ishida, Alcon Japan Ltd (F), AMO Japan K.K. (F), Bayer Yakuhin, Ltd. (F), Bonac Co., Ltd. (P), Chugai Pharmaceutical Co., Ltd. (P), Kowa Pharmaceutical Co., Ltd. (F), NIDEK Co., Ltd. (F), NIDEK Co., Ltd. (P), Novartis Pharma, K.K. (F), Otsuka Pharmaceutical Co., Ltd. (F), Santen Pharmaceutical Co., Ltd. (F), SEED Co., Ltd. (P), SEED Co., Ltd. (F), Senju Pharmaceutical Co., Ltd. (P), Senju Pharmaceutical Co., Ltd. (F), Takeyama Co., Ltd. (F), Wakamoto Pharmaceutical Co., Ltd. (F), White Medical Co.,Ltd. (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1123. doi:https://doi.org/
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      Kasumi Kikuchi, Kousuke Noda, Miyuki Murata, Yoshiaki Tagawa, Atsuhiro Kanda, Satoru Kase, Yasushi Kageyama, Masami Shinohara, Tomohiko Sasase, Susumu Ishida; Diabetic Cataract in Spontaneously Diabetic Torii Fatty Rats.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1123. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Spontaneously Diabetic Torii (SDT) fatty rat is a novel animal model of obese type 2 diabetes. Previously, it was shown that SDT fatty rats exhibited cataract formation as one of the diabetic ocular complications. However, time course of cataract development in SDT fatty rats has not been reported and its biochemical characteristics of the lens remain elusive. The aim of this study was to further investigate cataractogenesis in SDT fatty rats.

Methods : Male SDT fatty rats and age-matched SD rats (CLEA Japan, Inc.) were used. Body weight and blood glucose level were measured every week from 5 to 16 weeks of age. The time-course change of lens opacification was evaluated in 5 stages (stage 0: no cataract to 4: mature cataract) by a slit lamp examination. At 16 weeks of age, glucose and glutathione (GSH) concentrations were measured in the lens proteins.

Results : At 16 weeks of age, mean body weight of SDT fatty rats (n=13) and SD rats (n=11) were 526±9.8g and 485±3.8g, respectively (p<0.01). Blood glucose levels in SDT fatty rats (769±21mg/dl) were significantly higher than those in SD rats (108±4mg/dl, p<0.01). At 5 weeks of age, none of rats showed cataract formation; however, 12 SDT fatty rats (92.3%) developed mature cataract (stage 4) and the others (7.7%) showed severe cataract (stage 3) at 16 weeks of age. The lens GSH concentration was lower in SDT fatty rats (7.1±0.4nmol/mg) than SD rats (16.7±0.4nmol/mg, p<0.01), and the level of glucose was higher in the lens of SDT fatty rats (11.3±0.5nmol/mg) than that of SD rats (0.2±0.1nmol/mg, p<0.01).

Conclusions : The present data demonstrated that cataractogenesis in SDT fatty rats was consistent with that in patients with diabetes, indicating that SDT fatty rats have the potential to serve as an animal model of diabetic cataract associated with type 2 diabetes and obesity.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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