July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Role of the Matrix Metalloproteinase 10 (MMP-10) in Choroidal Neovascularization (CNV)
Author Affiliations & Notes
  • Maria Hernandez
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Patricia Fernandez
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Sergio Recalde
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Jose Antonio Rodriguez
    Atherothrombosis Laboratory. Cardiovascular Area, CIMA (Center for Applied Medical Research), Universidad de Navarra, Pamplona, Navarra, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Josune Orbe
    Atherothrombosis Laboratory. Cardiovascular Area, CIMA (Center for Applied Medical Research), Universidad de Navarra, Pamplona, Navarra, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Ana Montoliu
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
  • Jaione Bezunartea
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Idoia Belza
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
  • Elvira Rojas de Miguel
    Universidad de Navarra, Pamplona, Navarra, Spain
  • Jorge Gonzalez
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
  • Maite Moreno
    Universidad de Navarra, Pamplona, Navarra, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Elena Alonso
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Alfredo Garcia-Layana
    Laboratory of Experimental Ophthalmology, University of Navarra, Pamplona, Spain
    IdiSNA, Navarra Institute for Health Research, Pamplona, Navarra, Spain
  • Footnotes
    Commercial Relationships   Maria Hernandez, None; Patricia Fernandez, None; Sergio Recalde, None; Jose Rodriguez, None; Josune Orbe, None; Ana Montoliu, None; Jaione Bezunartea, None; Idoia Belza, None; Elvira Rojas de Miguel, None; Jorge Gonzalez, None; Maite Moreno, None; Elena Alonso, None; Alfredo Garcia-Layana, None
  • Footnotes
    Support  Spanish Multicenter Group on AMD and the “Red Temática de Investigación Cooperativa en Salud,” RD07/0062, OFTARED RD12/0034 and RD16/0008
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1249. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Maria Hernandez, Patricia Fernandez, Sergio Recalde, Jose Antonio Rodriguez, Josune Orbe, Ana Montoliu, Jaione Bezunartea, Idoia Belza, Elvira Rojas de Miguel, Jorge Gonzalez, Maite Moreno, Elena Alonso, Alfredo Garcia-Layana; Role of the Matrix Metalloproteinase 10 (MMP-10) in Choroidal Neovascularization (CNV). Invest. Ophthalmol. Vis. Sci. 2019;60(9):1249.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the role of metalloproteinase 10 (MMP-10) (stromelysin-2) in the development of choroidal neovascularization (CNV) process.

Methods : Laser-induced CNV was performed in adult MMP-10 deficient mice (n=12) and wt mice (n=5). The animals were sacrificed at 10 and 20 days postlaser. Fluorescent angiography (FA) and lectin staining was performed in retinal flat mounts. FA images were obtained and lectin images were captured by confocal microscope. The area of FA and CNV lesions was measured. Immunofluorescence was performed using MMP-10 antibody in MMP-10 deficient mice and wt.

Results : No changes in FA were observed. MMP-10 expression was absent in MMP-10 deficient mice flat mounts while in wt animals MMP-10 expression was observed in CNV areas. The reduction of the CNV area in MMP-10 deficient mice was statistically significant compared with control animals in early (47%, p=0.002) and advanced (67%, p=0.000) stages of the neovascularization process. T-student test for independent samples was applied and p<0.05 was considered statistically significant (SPSS v.15.0).

Conclusions : The present study support for the first time that MMP-10 is involved in the CNV formation in a laser-induced murine model suggesting the presence of an imbalance in the homeostasis of the extracellular matrix in processes of vascular pathology associated with inflammation, and altered vascular remodelling. These findings make us to consider this metalloproteinase as a pro-angiogenic molecule in the pathogenesis of Age-related macular degeneration (AMD). Therefore, MMP-10 could be considered for future alternative or combined therapies in pathological angiogenesis. However, further studies are needed to elucidate the involvement of MMP-10 in the development of AMD patients.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×