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Byron Li, Laisheng Jiang, Qin Liao, Zhijia Li, Zhaohui Yan; A Reanalysis of Laser–Induced Choroidal Neovascularization in the Cynomolgus Monkeys. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1260. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
The laser-induced non-human primate choroidal neovascularization (CNV) model has been used for many studies in this laboratory. Fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) data from these studies were reviewed to evaluate the grade 4 leakage lesion rate, the CNV incidence and the lesion self-healing rate of this model.
Data of 545 Cyno. monkeys (268M/277F), 2.5-5 yrs, were reviewed. On D1, laser lesions were induced bilaterally, 6-8 spots/eye, in the perimacular region (1.5-2 PD from fovea) with a 532 nm laser (spot size 50 µm, power 400-500 mW, exposure time 100 ms). The eyes were examined with FFA and OCT on days of 14, 21, 28, 35, 42, 49 and 56 (if available) post laser insult. On D14, lesions that developed early hyperfluorescence and late severe dye leakage beyond the border of the burn area during FFA were counted as grade 4 leakage lesion. The rate of grade 4 lesion (No. of grade 4 lesions / total No. of laser lesions x 100) and CNV incidence (No. of CNV eyes / total No. of lasered eyes x 100) were calculated. The subretinal hyperreflective material (SHRM) at each grade 4 lesion was imaged by OCT and its thickness was measured as well. The eyes in the control group of all studies were separately calculated for self-healing rate by comparing the average leakage area (ALA) of grade 4 lesions and the thickness of SHRM measured at each time point with that on D14.
Total 6888 laser burns were conducted in 1090 eyes (6.3 burns/eye). On D14, 75 eyes (40M/35F) failed to develop grade 4 leakage and 127 eyes (76M/51F) were removed from studies due to retinal hemorrhage. The grade 4 lesion rate was 60.1% (58.9% M / 61.2% F) and the CNV incidence was 81.4% (78.4% M / 84.5% F). Total 106 eyes were in the control group and their ALA and SHRM thickness of grade 4 lesions were gradually decreased along with the time. Compared with that measured on D14, the ALA was 98.3±43.6% and 91.2±51.1% on D21 and D28 (n=106), respectively. The rate was 104.1±13.1% on D35 (n=7), 77.4±26.2% on D42 (n=15), 66.4±13.8% on D49 (n=4) and 49.8±26.1% on D56 (n=4). Meanwhile, the average SHRM thickness of the same group was 92.0±13.1%, 86.2±17.5%, 83.6±8.5%, 79.6±13.9%, 79.2±12.0% and 67.6±10.3% at each time point.
This study provided useful information of laser-induced CNV monkey model. Especially, the lesion self-healing rate would help on regression study design.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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