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Jia Li, Baodi Deng, Xi Lu, Xiaoling Liang, Yue Xu; Estrogen receptors regulate angiogenesis in laser induced choroid neovascularization through Hif-1α/VEGF pathway.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1261.
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To investigate if estrogen receptor-α (ER-α), estrogen receptor-β (ER-β) and G-protein-coupled estrogen receptor-1 (GPER1) are expressed in laser induced choroid neovascularization in mice and reveal the role of these estrogen receptors in regulating angiogenesis process.
Choriod neovascularization was induced in mice using laser. Seven days after that, animals were euthanized and eyes were harvested. Immunofluorescence of choroid flat mounts and immunohistochemistry of paraffin sections were used to reveal the expression pattern of estrogen receptors. Quantitive PCR and westerblot were carried out to investigate the change in the expression of estrogen receptors, PHD2, Hif-1a, VEGF, VEGFr-2. Also，estrogen receptor agonist and inhibitor were used to demonstrate the role of estrogen receptors in regulating choroid neovascular growth. The size of choroid neovascularization was measured using OCT and IB4 stained choroid flat mounts. Leakage of new blood vessels was measured through FFA.
The G-protein-coupled estrogen receptor was found to express in laser induced CNV and the expression of GPER1, Hif-1α, VEGF, VEGFr-2 was significantly higher than the control group (p<0.05). Estrogen receptor inhibitor effectively suppressed the choroid new vessel growth.( p<0.05)
GPER1 is expressed in laser induced CNV in mice and regulate the growth of CNV through Hif-1α/VEGF pathway. Estrogen receptor inhibitor has the potential to be a novel treatment for CNV related eye diseases such as wet age related macular degeneration (wAMD).
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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