July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Fundus Autofluorescence Emission Spectra in ABCA4-related Retinopathy
Philipp L. Mueller; Chantal Dysli; Kristina Heß; Frank G Holz; Philipp Herrmann
Author Affiliations & Notes
  • Philipp L. Mueller
    Department of Ophthalmology, Universtity of Bonn, Bonn, Germany
  • Chantal Dysli
    Department of Ophthalmology, Universtity of Bonn, Bonn, Germany
    Department of Ophthalmology, University of Bern, Bern, Switzerland
  • Kristina Heß
    Department of Ophthalmology, Universtity of Bonn, Bonn, Germany
  • Frank G Holz
    Department of Ophthalmology, Universtity of Bonn, Bonn, Germany
  • Philipp Herrmann
    Department of Ophthalmology, Universtity of Bonn, Bonn, Germany
  • Footnotes
    Commercial Relationships   Philipp Mueller, Carl Zeiss MedicTec (F), CenterVue (F), Heidelberg Engineering (F), Optos (F); Chantal Dysli, Carl Zeiss MedicTec (F), CenterVue (F), Heidelberg Engineering (F), Optos (F); Kristina Heß, Carl Zeiss MedicTec (F), CenterVue (F), Heidelberg Engineering (F), Optos (F); Frank Holz, Acucela (F), Acucela (R), Allergan (F), Allergan (R), Appelis (F), Appelis (R), Bayer (F), Bayer (C), Bayer (R), Bioeq/Formycon (F), Bioeq/Formycon (C), Boehringer-Ingelheim (F), Carl Zeiss MedicTec (F), CenterVue (F), Ellex (F), Geuder (F), Grayburg Vision (F), Grayburg-Vision (R), Heidelberg Engineering (R), Heidelberg Engineering (C), Heidelberg Engineering (F), LinBioscience (F), Roche/Genentech (F), Roche/Genentech (R), Roche/Genentech (C); Philipp Herrmann, Carl Zeiss MedicTec (F), CenterVue (F), Heidelberg Engineering (F), Optos (F)
  • Footnotes
    Support  German Research Foundation Grant MU4279/1-1
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1332. doi:
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      Philipp L. Mueller, Chantal Dysli, Kristina Heß, Frank G Holz, Philipp Herrmann; Fundus Autofluorescence Emission Spectra in ABCA4-related Retinopathy. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1332.

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Abstract

Purpose : To investigate the characteristics of flecks in ABCA4-related retinopathy using different fundus autofluorescence (AF) excitation and emission spectra.

Methods : In this prospective monocenter study, a total of 120 eyes of 60 patients with genetically confirmed ABCA4-mutation were examined using multimodal AF imaging as well as spectral domain optical coherence tomography (OCT). AF imaging with blue light (BAF, 488 nm), green light (GAF, 518 nm), and near-infrared (NIR-AF, 787 nm) excitation wavelengths obtained by a confocal scanning laser ophthalmoscope (Spectralis HRA, Heidelberg Engineering, Heidelberg, Germany) was compared to a confocal light-emitting diode (LED) based retinal imaging system (EIDON, Centervue, Padova, Italy) with an excitation wavelength of 450 nm and fluorescence emission detection that allows for separation of short/green (GEFC, 500-560nm) and long/red (REFC, 560-700nm) fluorescence emission components.

Results : AF images with 450 nm, 488 nm and 518 nm excitation light revealed distinct distribution patterns of disease related flecks. In NIR-AF, hyperautofluorescent flecks were significant less frequent and distributed more eccentric. They highly correlated to flecks with predominantly REFC. Over an observation period of 5 to 14 months, new hyperautofluorescent flecks occurred that revealed predominantly REFC, while other flecks became hypoautofluorescent. Some flecks featured a loss of AF intensity in NIR-AF while staying hyperautofluorescent in the other AF modalities. These flecks showed a change from predominantly REFC to GEFC. In optical coherence tomography, the progression was associated with a disruption of outer retinal bands over the deposits.

Conclusions : By separation of AF emission spectra, a differentiated assessment of the composition of fluorophores and structural changes appears feasible within one single AF image. Change in the AF emission spectra over time might be a sensitive marker for disease progression and a potential new approach to assess therapeutic effects in future interventional trials.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright © 2025 Association for Research in Vision and Ophthalmology.
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