July 2019
Volume 60, Issue 9
ARVO Annual Meeting Abstract  |   July 2019
Influence of age and lens status on fluorescence lifetimes in FLIO
Author Affiliations & Notes
  • Chantal Dysli
    Ophthalmology, Hospital Bern Inselspital, Bern, Switzerland
  • Muriel Dysli
    Ophthalmology, Hospital Bern Inselspital, Bern, Switzerland
  • Sebastian Wolf
    Ophthalmology, Hospital Bern Inselspital, Bern, Switzerland
  • Martin Sebastian Zinkernagel
    Ophthalmology, Hospital Bern Inselspital, Bern, Switzerland
  • Footnotes
    Commercial Relationships   Chantal Dysli, None; Muriel Dysli, None; Sebastian Wolf, None; Martin Zinkernagel, None
  • Footnotes
    Support  Swiss national science foundation (SNSF) Nr 320030_156019
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1579. doi:
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      Chantal Dysli, Muriel Dysli, Sebastian Wolf, Martin Sebastian Zinkernagel; Influence of age and lens status on fluorescence lifetimes in FLIO. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1579.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose : Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) allows non-invasive in vivo measurement of autofluorescence lifetimes of retinal fluorophores upon excitation with blue laser light.
The aim of this study was to investigate age dependent autofluorescence lifetime changes in a large cohort of healthy eyes, and to compare phakic with pseudophakic subjects in order to obtain information about a possible influence of the crystalline lens on fluorescence lifetimes.

Methods : This study was conducted at the department of ophthalmology, University Hospital Bern, Switzerland. Fluorescence lifetime imaging was performed using a Fluorescence Lifetime Imaging Ophthalmoscope (Heidelberg Engineering, Heidelberg, Germany) with an excitation wavelength of 473 nm. Fluorescence decay times were measured in a short (498–560 nm, SSC) and in a long (560–720 nm, LSC) spectral channel. Individual fluorescence lifetime parameters were further investigated for both channels in phakic and pseudophakic subjects and correlated with age.

Results : Data of 141 healthy subjects (age: mean±SD 56 ± 18 years; range 21-91 years) were analysed. Retinal autofluorescence lifetimes in phakic eyes (82%) increases with age (SSC: r2= 0.54; r2= 0.7) by about 33 ps resp. 28 ps per decade. The short wavelengths channel between 498 and 560 nm was significantly influenced by the lens status, whereby pseudophakic subjects showed stable values without further increase with age. In the long wavelength channel between 560 and 720 nm, phakic and pseudophakic subjects featured a similar increase in lifetime with age.

Conclusions : Fluorescence lifetimes of the retina increase with age. Subclinical lens opacification mainly influences fluorescence lifetimes in the short spectral channel whereas in the long spectral channel no difference was observed between phakic and pseudophakic eyes.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.


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