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Anna Hillenmayer, Christian Wertheimer, Stefan Kassumeh, Claudia Priglinger, Siegfried Priglinger, Andreas Ohlmann; The role of lectines galectin-1 and 3 during the development of retinal vasculogenesis. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1645. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
Galectin-1 and 3 are ß-galactoside binding lectins with multiple functions such as regulation of immune response, angiogenesis and apoptosis. They influence cell-cell; cell-matrix interactions and modify signaling pathways. Galectin-1 and 3 could be detected in physiological and pathological retinal and choroidal vascular processes. However, their exact role in retinal angiogenesis remains unclear. We hypothesize that Galectin-1 and 3 modify the angiogenic potential of human retinal microvascular endothelial cells (HRMEC) in vitro. While moderate concentrations seem to have a proangiogenic influence on microvascular endothelial cells, high concentration lead to an inhibitory effect.
The effects of high concentrations of Galectin-1 and 3 on the HRMEC in vitro was researched using a cell proliferation BrdU Elisa and Wst-1 cell proliferation assay after incubation with 30; 60 and 120 µg/ml Galectin. The galectins’ influence on cell migration was investigated through a scratch-induced migration assay as well as cell migration tracking. Cell adhesion was measured. Tube formation was documented through an endothelial cell tube formation assay. Immunocytochemical staining was used for locating the lectins and detect modified pathways.
Incubation with high amounts of Galectin-1 and 3 lead to a dose-dependent decrease in cell proliferation, viability and cell migration compared to controls. They significantly reduced HRMEC in cell adhesion and tube formation.The staining located endogen Galectin-1 and 3 paranuclear, while incubation with exogen Galectin-1 lead to decreased cellular concentration and Galectin-3 to a separation and disposal through the formation of vesicles. The ß-Catenin Pathway was shown to be activated through exogen addition.
The results support our hypothesis that Galectin-1 and 3 directly influence and inhibit angiogenic activities of human retinal microvascular endothelial cell in high concentrations in vitro. Since endogen Galectins are shown to have a proangiogenic effect, further investigations will have to uncover the exact influential pathways for the reversion and possible therapeutic prospects.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.
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