July 2019
Volume 60, Issue 9
Open Access
ARVO Annual Meeting Abstract  |   July 2019
Japan Eye Genetics Consortium: National Cohort Survey and Whole Exome Sequencing Results
Author Affiliations & Notes
  • Kaoru Fujinami
    Ophthalmology, National Institute of Sensory Organs, Tokyo, TOKYO, Japan
    Genetics, UCL Institute of Ophthalmology, London, England, United Kingdom
  • Kazutoshi Yoshitake
    Ophthalmology, National Institute of Sensory Organs, Tokyo, TOKYO, Japan
  • Takaaki Hayashi
    The Jikei University School of Medicine, Japan
  • Kazuki Kuniyoshi
    Kinki University Faculty of Medicine, Osaka-Sayama, Osaka, Japan
  • Shinji Ueno
    Nagoya University Graduate School of Medicine, Aichi, Japan
  • Mineo Kondo
    Mie Univ Graduate School of Medicine, Tsu, Mie, Japan
  • Kei Shinoda
    Teikyo University School of Medicine, Japan
  • Shuhei Kameya
    Nippon Medical School Chiba Hokusoh Hospital, Japan
  • Nobuhisa Nao-i
    School of Medicine, Miyazaki University, Japan
  • Hiroyuki Kondo
    University of Occupational and Environmental Health, Japan
  • Yozo Miyake
    Aichi Medical University, Japan
  • Takeshi Iwata
    Ophthalmology, National Institute of Sensory Organs, Tokyo, TOKYO, Japan
  • Kazushige Tsunoda
    Ophthalmology, National Institute of Sensory Organs, Tokyo, TOKYO, Japan
  • Footnotes
    Commercial Relationships   Kaoru Fujinami, Acucela Inc. (C), Astellas Pharma Inc (C), Astellas Pharma Inc (F), Foundation Fighting Blindness, (F), Foundation Fighting Blindness Clinical Research Institute, (I), Japanese Ophthalmology Society (I), Japan Retinitis Pigmentosa Society (I), Kubota Pharmaceutical Holdings Co., Ltd. (C), NightstaRx Limited. (F), Novartis International AG (F), SANTEN Company Limited (I); Kazutoshi Yoshitake, None; Takaaki Hayashi, None; Kazuki Kuniyoshi, None; Shinji Ueno, None; Mineo Kondo, None; Kei Shinoda, None; Shuhei Kameya, None; Nobuhisa Nao-i, None; Hiroyuki Kondo, None; Yozo Miyake, None; Takeshi Iwata, None; Kazushige Tsunoda, None
  • Footnotes
    Support  Grant-in-Aid for Young Scientists (A) of the Ministry of Education, Culture, Sports, Science and Technology, Japan (16H06269), grants from Grant-in-Aid for Scientists to support international collaborative studies of the Ministry of Education, Culture, Sports, Science and Technology, Japan (16KK01930002), grants from National Hospital Organization Network Research Fund (H30-NHO-Sensory Organs-03), grants from FOUNDATION FIGHTING BLINDNESS ALAN LATIES CAREER DEVELOPMENT PROGRAM (CF-CL-0416-0696-UCL), grants from Health Labour Sciences Research Grant, The Ministry of Health Labour and Welfare (201711107A), and grants from Great Britain Sasakawa Foundation Butterfield Awards.
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1758. doi:
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    • Get Citation

      Kaoru Fujinami, Kazutoshi Yoshitake, Takaaki Hayashi, Kazuki Kuniyoshi, Shinji Ueno, Mineo Kondo, Kei Shinoda, Shuhei Kameya, Nobuhisa Nao-i, Hiroyuki Kondo, Yozo Miyake, Takeshi Iwata, Kazushige Tsunoda; Japan Eye Genetics Consortium: National Cohort Survey and Whole Exome Sequencing Results. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1758.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We describe the clinical and genetic characteristics of a nationwide cohort with inherited retinal/choroidal diseases (IRD) in Japanese population.

Methods : A cohort of 1430 affected and 686 unaffected subjects from 1247 Japanese families with IRD was ascertained between 2008 and 2018. The clinical diagnosis was performed with comprehensive ophthalmic examinations at 38 institutes throughout the nation. The cohort included retinitis pigmentosa (RP; 564 families), macular dystrophy or cone (-rod) dystrophy (MD/CORD; 167 families), occult macular dystrophy (OMD; 106 families), Stargardt disease (STGD; 68 families), Leber congenital amaurosis (LCA; 63 families), CORD with normal fundus (CORD-NF; 54 families), cone dysfunction syndrome (CDS, 51 families)congenital stationary night blindness (CSNB; 40 families), and others. Whole exome sequencing and molecular genetic analysis was performed in 829 affected and 427 unaffected subjects from 735 families.

Results : Disease-causing variants were determined in 373/735 (50.7%) families, including 235 (235/373, 63.0%) with previously reported variants and 138 families (235/373, 37.0%) with novel variants. In total 629 detected variants, 459 (459/629, 73.0%) have been previously reported and 170 (170/629, 27.0%) are novel variants of previously reported genes. No definitive disease-causing variants or variants of putative new associated genes never reported were found in 362/735 (362/735, 49.3%) families. The disease-causing variants were determined in 159 of 287 (159/287, 55.4%) families with RP, 51 of 93 (51/92, 54.8%) families with MD/CORD, 32 of 75 (32/75, 42.7%) families with OMD, 19 of 43 (19/43, 44.2%) families with LCA, 14 of 45 (14/45, 31.3%) families with CORD-NF, 16 of 35 (16/35, 45.7%) families with CDS, and 16 of 23 (13/23, 56.5%) families with CSNB.

Conclusions : Conclusive molecular genetic diagnosis is obtained in half of this Japanese IRD cohort. Novel disease-causing variants are frequently revealed, which suggests the distinctive genetic background of Japanese population. This national survey provides an epidemiologic evidence of Japanese IRD, which promotes patients’ care and therapeutic trials in Japanese population.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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