July 2019
Volume 60, Issue 9
Free
ARVO Annual Meeting Abstract  |   July 2019
Structural Correlates of Short-wavelength and Normalized Near-infrared Fundus Autofluorescence Imaging in Choroideremia.
Author Affiliations & Notes
  • Drew Scoles
    Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
  • Kurt Scavelli
    Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
  • Leona Serrano
    Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
    Center for Advanced Retinal and Ophthalmic Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
  • Katherine E Uyhazi
    Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
    Center for Advanced Retinal and Ophthalmic Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
  • Tomas S Aleman
    Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
    Center for Advanced Retinal and Ophthalmic Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Drew Scoles, None; Kurt Scavelli, None; Leona Serrano, None; Katherine Uyhazi, None; Tomas Aleman, None
  • Footnotes
    Support  National Institutes of Health (NEI-K12EY015398-10), Research to Prevent Blindness, Foundation Fighting Blindness, Hope for Vision, Macula Vision Research, the Paul and Evanina Bell Mackall Foundation Trust and The Pennsylvania Lions Sight Conservation
Investigative Ophthalmology & Visual Science July 2019, Vol.60, 1864. doi:
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    • Get Citation

      Drew Scoles, Kurt Scavelli, Leona Serrano, Katherine E Uyhazi, Tomas S Aleman; Structural Correlates of Short-wavelength and Normalized Near-infrared Fundus Autofluorescence Imaging in Choroideremia.. Invest. Ophthalmol. Vis. Sci. 2019;60(9):1864.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To better understand the origin and pattern of fundus autofluorescence (FAF) elicited by near-infrared (NIR) and short-wavelength (SW) excitation lights in subjects with choroideremia (CHM). To assess the utility of normalization of the NIR-FAF by the strength of the NIR reflectance (NIR-REF) signal.

Methods : Previously obtained imaging of 10 subjects (20 eyes) with CHM ages 11-60 years old at various disease stages from two sessions (6 to 29 months apart, average 16) was analyzed. 6 eyes had been previously treated as part of a gene therapy trial. The NIR-REF, SW-FAF, and NIR-FAF as well as the spectral domain optical coherence tomography (SD-OCT) images from each time point were co-registered in a semi-automated fashion. The normalized NIR-FAF (nNIR-FAF) was calculated as previously described by Cideciyan et al. A pixel value profile across the path of the horizontal SD-OCT cross section was extracted from each of the fundus imaging modalities. The horizontal extents of the islands of FAF were manually marked.

Results : nNIR-FAF provides increased relative contrast of retained islands of retinal pigment epithelium (RPE) compared to NIR-FAF, but was less than SW-FAF. In all but one subject the horizontal extent of SW-FAF and nNIR-FAF was well co-localized. SW-FAF and nNIR-FAF islands corresponded to areas with relatively preserved ellipsoid zone and RPE bands on SD-OCT. nNIR-FAF in 7 out of 10 subjects showed a wide parafoveal peak of autofluorescence which corresponded with regions of relatively preserved outer segment length. Both eyes of one young subject with early disease demonstrated annular parafoveal SW-FAF preservation surrounded by perifoveal mottled and reduced SW-FAF. There was no clear correlate to this annulus with nNIR-FAF. Corresponding OCT showed loss of interdigitation zone with preservation of other outer retinal bands.

Conclusions : Changes in NIR and SW-FAF represent structural changes of the outer retina as seen SD-OCT. These changes are well co-localized between these two modalities in most cases, however, with much lower contrast using NIR excitation especially in the setting of poor image quality. nNIR-FAF modality provides improved contrast, facilitating identification of preserved NIR-FAF. With optimized imaging conditions it may be possible to forego SW-FAF and thus spare subjects the associated discomfort and potential photochemical damage.

This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.

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