Abstract
Purpose :
we assessed the quality, quantity, and safety of layer-by-layer self-assembly by examining their morphology, function, immunogenicity in vitro and vivo.
Methods :
We have used layer-by-layer self-assembly as a suitable delivery carrier for cells, it has been suggested that layer-by-layer assembled cells by gelatin and alginate may represent a useful cells transplatation and factors delivery therapies to improve cells survival. In the present study, we established layer-by-layer assembled RPE cells by gelatin and alginate to assess survival rate, immunogenicity, and cellular function in vitro and in vivo.
Results :
The results of mixed lymphocyte reactions, IFN-γ ELISIA, and T cells rejection assay suggested that LbL-RPE cells had a lower immunogenicity. Additionally, upon transplantation, Iba1+cells, MHC class II+cells, and CD3+ T cells pooly invaded the LbL-RPE cells area by immunohistochemical examination, and ERG recordings also showed higher amplitude of B wave in LbL-RPE cells group. These LbL-RPE implants were capable of preventing cell dispersion, reducing the immunogenicity, and saving the vision without any immunosuppression during 5-week observation.
Conclusions :
we demonstrated self-assembled gelatin and alginate coated RPE cells that can enhance RPE cells attachment, survival, and function, we have used an optimization strategy to decrease immunogenicity in the RPE cells transplantation to save the vision.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.