Abstract
Purpose :
Vitreous play important roles in eye development, supporting posterior segment structures and transporting oxygen and nutrients. However, ocular features of vitreous cavity and vitreous diseases via Optical Coherence Tomography (OCT) imaging are few reported. Using persistent hyperplastic primary (PHPV) as a vitreous disease model, our study is to identify imaging characteristics of mouse PHPV by Spectralis OCT, as well as to assess and compare the sensitivity and precision of OCT with color photography and Fundus Fluorescein Angiography (FFA) imaging in detecting mouse PHPV.
Methods :
C57BL/6J mouse (224 eyes) aged from 3 months to 7 months were examined in this study. Color photography, FFA and OCT imaging were utilized to examine vitreous cavity and retina of mouse eyes. Horizontal and radial OCT scan volume was centered on the optic nerve head. Hematoxylin and eosin (H&E) staining was performed to validate PHPV.
Results :
For color photography and FFA imaging, retrolental irregular fibrovascular membrane-like tissues were found in 33 eyes with/without blood vessels in vitreous cavity. Among them, 31 eyes were visualized with lateral and oblique linear hyperreflective opacities in vitreous cavity using Spectralis OCT. Position of PHPV in posterior segment of eyes was also measured via OCT. Mouse PHPV was validated by H&E staining.
Conclusions :
In our studies, lateral and oblique linear hyperreflective opacities in vitreous cavity were visualized in PHPV mouse. OCT correlated well with color photography and FFA imaging in detecting mouse PHPV. OCT scanning can also be used to detect the location of PHPV in mouse eyes. The vitreoretinal relationship and fine details can be clearly visualized using OCT and PHPV mouse models. Our study will lead to a better understanding of vitreous disease and contribute to the development of OCT application in human vitreous examination.
This abstract was presented at the 2019 ARVO Annual Meeting, held in Vancouver, Canada, April 28 - May 2, 2019.